Several large or mid-scale collections of Drosophila enhancer traps have been recently created to allow for genetic swapping of GAL4 coding sequences to versatile transcription activators or suppressors such as LexA, QF, split-GAL4 (GAL4-AD and GAL4-DBD), GAL80 and QS. Yet a systematic analysis of the feasibility and reproducibility of these tools is lacking. Here we focused on InSITE GAL4 drivers that specifically label different subpopulations of olfactory neurons, particularly local interneurons (LNs), and genetically swapped the GAL4 domain for LexA, GAL80 or QF at the same locus. We found that the major utility-limiting factor for these genetic swaps is that many do not fully reproduce the original GAL4 expression patterns. Different donors exhibit distinct efficacies for reproducing original GAL4 expression patterns. The successfully swapped lines reported here will serve as valuable reagents and expand the genetic toolkits of Drosophila olfactory circuit research.

最近建立了几个果蝇增强子陷阱的大型或中型集合，以允许将GAL4编码序列遗传交换到通用的转录激活剂或抑制子，例如LexA，QF，split-GAL4（GAL4-AD和GAL4-DBD），GAL80和QS。但是，缺乏对这些工具的可行性和可重复性的系统分析。在这里，我们重点介绍InSITE GAL4专门标记嗅觉神经元的不同亚群（尤其是局部中间神经元（LNs））的驱动程序，并在同一位置将GAL4结构域遗传交换为LexA，GAL80或QF。我们发现，这些基因交换的主要效用限制因素是许多基因不能完全复制原始的GAL4表达模式。不同的供体表现出独特的复制原始GAL4表达模式的功效。此处报道的成功交换品系将用作有价值的试剂，并扩展果蝇嗅觉回路研究的遗传工具包。