After decades of research and development, synthetic nucleic acids are beginning to enjoy significant success in the clinic. Approved drugs have increased interest in the field, and many basic research studies have focused on synthetic nucleic acids to control the action of messenger RNA and noncoding RNAs. Unfortunately, experimental designs are often inadequate, resulting in misleading interpretation of data and unconvincing work that wastes resources and does little to advance the field. The goal of this commentary is to outline the problems facing many researchers, especially those new to the use of synthetic oligonucleotides. We describe the minimum control experiments necessary to build a strong case for real effects that are likely due to interactions at the intended molecular target. A common set of standards for preparing and judging experiments should facilitate better interpretation of data and publications that contribute positively to using synthetic nucleic acids as tools and drugs.

中文翻译：

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使用反义寡核苷酸和双链RNA的实验指南。

经过数十年的研究和开发，合成核酸已开始在临床上取得巨大成功。批准的药物在该领域的兴趣日益增加，许多基础研究已集中在合成核酸上，以控制信使RNA和非编码RNA的作用。不幸的是，实验设计常常是不充分的，导致对数据的误解和令人信服的工作，这浪费了资源，对推进该领域几乎没有帮助。本评论的目的是概述许多研究人员面临的问题，尤其是使用合成寡核苷酸的新问题。我们描述了必要的最低限度的控制实验，以建立有力的案例来证明可能由于预期分子靶标之间的相互作用而产生的真实效果。