This work studied the effect of deoxynivalenol (DON) and two of its acetylated analogs (3-ADON, 15-ADON) on first indicators of oxidative stress status, namely production of reactive oxygen species (ROS) and induction of lipid peroxidation (LPO), in HepG2 cells. HepG2 cells were exposed to different concentrations of the three toxins, either alone or in combinations, for 24, 48, and 72 h. Results of cytotoxicity obtained in HepG2 cells were correlated with the detection of ROS and LPO. This effect was inversely correlated with ROS while directly correlated with LPO for the assayed mycotoxins in individual treatment. Combinations of two toxins containing 15-ADON yielded highest values, while for two-toxin combinations with 3-ADON, the effects were minor. A combination of all three mycotoxins alleviated ROS production and the highest levels in LPO were detected, in association to a final breakdown of adaption of ROS early produced by HepG2. In conclusion, parameters of stress evaluation presented in this study (ROS and LPO), revealed increases in HepG2 cells exposed to DON, 3-ADON, and 15-ADON either individually or combined.

中文翻译：

---

脱氧雪茄烯醇，3-乙酰基-脱氧雪茄烯醇和15-乙酰基-脱氧雪茄烯醇对HepG2细胞氧化应激相关参数的影响。

这项工作研究了脱氧雪腐烯醇（DON）及其两个乙酰化类似物（3-ADON，15-ADON）对氧化应激状态的第一个指标的影响，即活性氧（ROS）的产生和脂质过氧化的诱导（LPO） ，在HepG2细胞中。将HepG2细胞单独或组合暴露于不同浓度的三种毒素中24、48和72小时。在HepG2细胞中获得的细胞毒性结果与ROS和LPO的检测相关。对于单个治疗中测定的霉菌毒素，该作用与ROS呈负相关，而与LPO直接相关。包含15-ADON的两种毒素的组合产生最高值，而对于包含3-ADON的两种毒素的组合，影响很小。所有这三种霉菌毒素的组合均可降低ROS的产生，并检测到LPO的最高水平，这与HepG2早期产生的ROS适应性的最终分解有关。总之，本研究中提出的压力评估参数（ROS和LPO）表明，单独暴露或联合暴露于DON，3-ADON和15-ADON的HepG2细胞增加。