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Analysis of historical negative control group data from the in vitro micronucleus assay using human lymphocytes.
Mutation Research/Genetic Toxicology and Environmental Mutagenesis ( IF 1.9 ) Pub Date : 2019-01-01 , DOI: 10.1016/j.mrgentox.2018.08.009
David P Lovell 1 , Mick Fellow 2 , Azeddine Elhajouji 3 , Christopher S Farabaugh 4 , Ben G Gilby 5 , Kiyohiro Hashimoto 6 , Yan Li 7 , Shambhu Roy 8 , Maik Schuler 9 , James Whitwell 10 , Jennifer Y Tanir 11
Affiliation  

A database of the micronuclei counts was built up for historical negative control data from human lymphocyte in vitro micronuclei tests (MnVit) carried out in 8 laboratories with experience of the method. The mean incidence of micronucleated cells (mnt)/1000 cells ranged from 2.2/1000 to 15.9/1000. There were no large differences in incidence between the presence or absence of S9 mix or between different treatment lengths. There was also little evidence that different solvents affected the numbers of micronuclei appreciably. A number of laboratories did show significant inter-experiment variability, indicating that there remained unidentified factors affecting frequencies. Donor variance may be one such factor. Inter-individual variability may explain some of these differences. The approximate 7.5-fold difference in mnt/1000 scores in a relatively small group of experienced laboratories illustrates the potential complications that can arise if a metric like a fold increase was considered the only biologically important finding. Although there is inherent variability between experiments, it was evident that within a laboratory the overall laboratory mean remains constant over time. It is believed that these findings will provide help to laboratories conducting studies using human lymphocytes in the MnVit and to those involved in the assessment of MnVit results.

中文翻译:

使用人淋巴细胞进行的体外微核分析对阴性对照组的历史数据进行了分析。

建立了微核计数数据库,以获取来自人类淋巴细胞体外微核试验(MnVit)的历史阴性对照数据,该试验在8个实验室中进行,具有该方法的经验。微核细胞(mnt)/ 1000细胞的平均发生率在2.2 / 1000至15.9 / 1000之间。在存在或不存在S9混合物之间或不同治疗时间之间,发病率差异不大。也几乎没有证据表明不同的溶剂会明显影响微核的数量。许多实验室确实显示出明显的实验间差异,表明仍然存在影响频率的不确定因素。供体差异可能是这样的因素之一。个体间的变异性可以解释其中的一些差异。大约7。在相对较小的一组经验丰富的实验室中,mnt / 1000得分的5倍差异说明,如果将像增加倍数这样的指标视为唯一的生物学重要发现,则可能会出现潜在的并发症。尽管实验之间存在固有的可变性,但很明显,在实验室内,整个实验室的平均值随时间保持不变。相信这些发现将为使用MnVit中的人类淋巴细胞进行研究的实验室以及参与MnVit结果评估的实验室提供帮助。显然,在实验室内,整个实验室的平均值在一段时间内保持不变。相信这些发现将为使用MnVit中的人类淋巴细胞进行研究的实验室以及参与MnVit结果评估的实验室提供帮助。显然,在实验室内,整个实验室的平均值在一段时间内保持不变。相信这些发现将为使用MnVit中的人类淋巴细胞进行研究的实验室以及参与MnVit结果评估的实验室提供帮助。
更新日期:2019-11-01
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