Pesticides are one of the most frequently investigated chemical, due to their multiple uses in agricultural and public health areas. This study evaluates lymphocytes CBMN (cytokinesis-block micronucleus cytome assay), inflammatory markers, inorganic elements in blood samples, and the relationship of these parameters with XRCC1Arg194Trp, OGG1Ser326Cys and PON1Gln192Arg polymorphisms in a population of tobacco farmers. The study population comprised 129 agricultural workers exposed to pesticides and 91 nonexposed. Farmers had significantly increased NPB (nuclear plasmatic bridge), MN (micronucleus) and NBUD (nuclear bud) frequencies, as well as IL-6 (interleukin 6) and TNF-α (tumor necrosis factor alpha) serum levels, and decreased cytokines CD4+/CD8+ ratio. In the exposed group, XRCC1 Trp/- was correlated with decreased NDI (nuclear division index), and OGG1 Cys/- was associated with higher levels of NPB and decreased levels of IL-6. The combined effects of PON1 Arg/- and XRCC1 Arg/Arg were associated with increased NPB frequencies. In addition, the combination of PON1 Arg/- with XRCC1 Trp/- or OGG1 Cys/- influenced in increased levels of necrosis in farmers. Furthermore, tobacco farmers showed a positive correlation between TNF-α levels and NPB, CD4+/CD8+ ratio and NBUD; and IL-6 levels with both MN and NDI. The duration of years of work at tobacco fields was correlated positively with NBUD frequency. Sulfur, chlorine and potassium were found at increased levels in the exposed group when compared to the nonexposed one. These findings provide evidence that tobacco farmers' exposure have increased DNA damage and alter the immune system's response, and that XRCC1 and OGG1 polymorphisms could influence both biomarkers results.

中文翻译：

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碱基切除修复（OGG1和XRCC1）和新陈代谢（PON1）基因多态性作用于调节烟农的DNA损伤和免疫参数。

由于农药在农业和公共卫生领域有多种用途，因此农药是研究最频繁的化学品之一。这项研究评估了烟农群体中淋巴细胞CBMN（胞质阻滞性微核细胞计数），炎性标志物，血样中的无机元素以及这些参数与XRCC1Arg194Trp，OGG1Ser326Cys和PON1Gln192Arg多态性之间的关系。该研究人群包括129位暴露于农药的农业工人和91位未暴露的农业工人。农民的NPB（核浆桥），MN（微核）和NBUD（核芽）频率以及IL-6（白介素6）和TNF-α（肿瘤坏死因子α）血清水平显着升高，而细胞因子CD4 +降低/ CD8 +比率。在暴露组中，XRCC1 Trp /-与NDI（核分裂指数）降低相关，OGG1 Cys /-与更高的NPB水平和降低的IL-6水平相关。PON1 Arg /-和XRCC1 Arg / Arg的组合效应与NPB频率增加有关。另外，PON1 Arg /-与XRCC1 Trp /-或OGG1 Cys /-的结合也影响了农民的坏死水平。此外，烟农显示TNF-α水平与NPB，CD4 + / CD8 +比率和NBUD之间呈正相关。MN和NDI的IL-6水平。烟草田的工作年限与NBUD频率呈正相关。与未暴露组相比，暴露组中的硫，氯和钾含量增加。这些发现提供了证据，证明烟农的暴露增加了DNA损伤并改变了免疫系统的反应，