We herein describe a personal glucose meter (PGM)-based method for a label-free and washing-free determination of alkaline phosphatase (ALP) activity, which relies on the cascade enzymatic reactions promoted by hexokinase and pyruvate kinase to couple ALP activity with the amount of glucose. In principle, the presence of target ALP scavenges on adenosine 5'-triphosphate (ATP), a phosphate source for hexokinase-catalyzed reactions, and thus suppresses the ensuing cascade enzymatic reactions. As a result, the initial high amount of glucose is maintained and the amount of glucose, which is proportional to ALP activity, is simply measured by a hand-held PGM. Based on this novel strategy, we successfully determined the ALP activity down to 8.9 U/L with the high selectivity. In addition, the diagnostic capability of this method was demonstrated by reliably assaying the ALP activity in non-diluted human blood without any pretreatment steps.

中文翻译：

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使用个人血糖仪进行无标记和免清洗的碱性磷酸酶测定。

我们在本文中介绍了一种基于个人血糖仪（PGM）的无标记和免洗碱性磷酸酶（ALP）活性测定方法，该方法依赖于己糖激酶和丙酮酸激酶促进的级联酶促反应，使ALP活性与酶结合。葡萄糖量。原则上，目标ALP的存在会清除己糖激酶催化反应的磷酸根源5'-三磷酸腺苷（ATP），从而抑制随后的级联酶促反应。结果，维持了最初的高葡萄糖量，并且与葡萄糖酸水平成正比的葡萄糖量可以通过手持式PGM进行简单测量。基于这种新颖的策略，我们以高选择性成功地确定了低至8.9 U / L的ALP活性。此外，