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The Impact of Pyroglutamate: Sulfolobus acidocaldarius Has a Growth Advantage over Saccharolobus solfataricus in Glutamate-Containing Media.
Archaea ( IF 2.4 ) Pub Date : 2019-04-24 , DOI: 10.1155/2019/3208051
Anna M Vetter 1 , Julia Helmecke 1 , Dietmar Schomburg 1 , Meina Neumann-Schaal 1, 2
Affiliation  

Microorganisms are well adapted to their habitat but are partially sensitive to toxic metabolites or abiotic compounds secreted by other organisms or chemically formed under the respective environmental conditions. Thermoacidophiles are challenged by pyroglutamate, a lactam that is spontaneously formed by cyclization of glutamate under aerobic thermoacidophilic conditions. It is known that growth of the thermoacidophilic crenarchaeon Saccharolobus solfataricus (formerly Sulfolobus solfataricus) is completely inhibited by pyroglutamate. In the present study, we investigated the effect of pyroglutamate on the growth of S. solfataricus and the closely related crenarchaeon Sulfolobus acidocaldarius. In contrast to S. solfataricus, S. acidocaldarius was successfully cultivated with pyroglutamate as a sole carbon source. Bioinformatical analyses showed that both members of the Sulfolobaceae have at least one candidate for a 5-oxoprolinase, which catalyses the ATP-dependent conversion of pyroglutamate to glutamate. In S. solfataricus, we observed the intracellular accumulation of pyroglutamate and crude cell extract assays showed a less effective degradation of pyroglutamate. Apparently, S. acidocaldarius seems to be less versatile regarding carbohydrates and prefers peptidolytic growth compared to S. solfataricus. Concludingly, S. acidocaldarius exhibits a more efficient utilization of pyroglutamate and is not inhibited by this compound, making it a better candidate for applications with glutamate-containing media at high temperatures.

中文翻译:

焦谷氨酸的影响:在含谷氨酸盐的培养基中,嗜酸小球菌比Solcharotbus solfataricus具有增长优势。

微生物非常适合其栖息地,但对其他生物体分泌的或在相应环境条件下化学形成的有毒代谢物或非生物化合物部分敏感。嗜热嗜酸菌受到焦谷氨酸的挑战,焦内酰胺是在有氧嗜热嗜酸条件下通过将谷氨酸环化而自发形成的内酰胺。众所周知,焦谷氨酸可以完全抑制嗜热嗜酸性克杆菌Saccharolobus solfataricus(以前称为Sulfolobus solfataricus)的生长。在本研究中,我们调查了焦谷氨酸对S. solfataricus和紧密相关的crenarchaeon Sulfolobus acidocaldarius生长的影响与之相反以焦谷氨酸为唯一碳源成功地培育了S. solfataricusS。acidocaldarius。生物信息学分析表明,磺科的两个成员都具有至少一种5-氧代脯氨酸酶的候选物,该酶可催化焦磷酸谷氨酸向谷氨酸的ATP依赖性转化。在S. solfataricus中,我们观察到了焦谷氨酸的细胞内积累,粗细胞提取物检测显示焦谷氨酸的降解效果较差。显然,S.热硫化叶菌似乎是通用性较差有关碳水化合物,喜欢比较肽分解成长S.叶菌。最后,嗜酸链球菌 表现出更有效的焦谷氨酸利用,并且不受该化合物的抑制,因此使其更适合在高温下与含谷氨酸的培养基一起使用。
更新日期:2019-04-24
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