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Modified Hiraoka TEM grid staining apparatus and technique using 3D printed materials and gadolinium triacetate tetrahydrate, a nonradioactive uranyl acetate substitute
Journal of Histotechnology ( IF 1.1 ) Pub Date : 2017-08-11 , DOI: 10.1080/01478885.2017.1361117
Philip Seifert 1
Affiliation  

Abstract The Hiraoka transmission electron microscopy (TEM) grid staining apparatus, which allowed multiple grids to be batch stained together on a plate, is no longer commercially available. Because the need for such a device still exists, we developed a modified Hiraoka TEM grid staining apparatus by combining a Leica AC20 grid plate holder with a modified 3D printed grid loading holder and trays lined with ParafilmR. This apparatus was then used to test a post-section contrasting method that uses gadolinium triacetate tetrahydrate solution, a nonradioactive uranyl acetate substitute, which produces similar staining results to uranyl acetate when used with lead stain in mouse ocular tissue for TEM.

中文翻译:

使用 3D 打印材料和四水合三乙酸钆(一种非放射性乙酸铀酰替代品)改进的 Hiraoka TEM 网格染色装置和技术

摘要 平冈透射电子显微镜 (TEM) 网格染色装置允许在一块板上对多个网格进行批量染色,但该装置已不再市售。由于对这种设备的需求仍然存在,我们通过将 Leica AC20 网格板支架与改进的 3D 打印网格装载支架和衬有 ParafilmR 的托盘相结合,开发了一种改进的 Hiraoka TEM 网格染色装置。然后,该装置用于测试切片后对比方法,该方法使用三乙酸钆四水合物溶液(一种非放射性乙酸双氧铀替代品),当与小鼠眼组织中的铅染色一起用于 TEM 时,可产生与乙酸双氧铀类似的染色结果。
更新日期:2017-08-11
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