Cellular migration is essential in diverse physiological and pathophysiological processes. Here, we present a protocol for quantitative analysis of migration using confluence detection allowing continuous, non-endpoint measurement with minimal hands-on time under cell incubator conditions. Applicability was tested using substances which enhance (EGF) or inhibit (cytochalasin D, ouabain) migration. Using a gap-closure assay we demonstrate that automated confluence detection monitors cellular migration in the 96-well microplate format. Quantification by % confluence, % cell free-area or % confluence in cell-free area against time, allows detailed analysis of cellular migration. The study describes a practicable approach for continuous, non-endpoint measurement of migration in 96-well microplates and for detailed data analysis, which allows for medium/high-throughput analysis of cellular migration in vitro.

中文翻译：

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使用融合测量，连续，无标记，基于96孔的细胞迁移测定。

细胞迁移在多种生理和病理生理过程中至关重要。在这里，我们介绍了一种使用汇合检测对迁移进行定量分析的方案，该方案允许在细胞培养箱条件下以最少的动手时间进行连续的非终点测量。使用增强（EGF）或抑制（细胞松弛素D，哇巴因）迁移的物质测试其适用性。使用间隙封闭测定法，我们证明了自动融合检测可监测96孔微孔板形式的细胞迁移。通过对汇合百分比，无细胞面积百分比或无细胞面积汇合百分比随时间的量化，可以对细胞迁移进行详细分析。这项研究描述了一种可行的方法，用于连续，无终点地测量96孔微孔板中的迁移以及进行详细的数据分析，