This study aimed to clarify relationships among UDP-glucose-6 dehydrogenase (UGDH) expression, clinicopathological factors, and the prognosis of patients, and to determine the role of UGDH in lung adenocarcinoma (AC). Firstly, UGDH expression and localization in 126 lung AC tissues were immunohistochemically studied, and associations with clinicopathological parameters and patients' prognosis were evaluated. Secondly, serum UGDH levels were measured in 267 lung cancer patients and 100 healthy controls. Finally, the effects of UGDH knockdown by siRNA on migration and invasion abilities were analyzed. As a result, nuclear UGDH staining was significantly correlated with poorer differentiation, a larger tumor size, higher p-TNM stage, positive nodal metastasis, positive lymphatic invasion, and positive vascular invasion in lung AC patients. Nuclear UGDH-positive patients showed significantly poorer survival than nuclear UGDH-negative patients. Serum UGDH levels were especially higher in lung AC patients even in stage I than those in healthy controls. In lung AC cell lines, nuclear expression levels of UGDH were higher in LC-2/ad cells than in A549 cells. UGDH siRNA-treated LC-2/ad cells showed significantly decreased migration and invasion abilities, but no significant differences were observed in UGDH siRNA-treated A549 cells. These data indicate that UGDH expression and localization are an early sero-diagnostic marker in addition to a poor prognostic indicator in lung AC patients.

中文翻译：

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UGDH核定位在肺腺癌中的临床病理和预后意义。

本研究旨在阐明UDP-葡萄糖-6脱氢酶（UGDH）的表达，临床病理因素和患者预后之间的关系，并确定UGDH在肺腺癌（AC）中的作用。首先，免疫组化研究了UGDH在126例肺AC组织中的表达和定位，并评估了其与临床病理参数和患者预后的关系。其次，在267例肺癌患者和100例健康对照者中测量了血清UGDH水平。最后，分析了siRNA UGDH敲低对迁移和侵袭能力的影响。结果，肺AC患者的UGDH核染色与较差的分化，较大的肿瘤大小，较高的p-TNM分期，阳性淋巴结转移，阳性淋巴管浸润和阳性血管浸润相关。核UGDH阳性患者的存活率显着低于核UGDH阴性患者。甚至在I期，在AC肺部AC患者中血清UGDH水平也特别高于健康对照组。在肺AC细胞系中，LC-2 / ad细胞中UGDH的核表达水平高于A549细胞。UGDH siRNA处理的LC-2 / ad细胞显示出明显降低的迁移和侵袭能力，但在UGDH siRNA处理的A549细胞中未观察到显着差异。这些数据表明，在肺AC患者中，除了不良的预后指标外，UGDH的表达和定位是早期的血清诊断指标。甚至在I期，在AC肺部AC患者中血清UGDH水平也特别高于健康对照组。在肺AC细胞系中，LC-2 / ad细胞中UGDH的核表达水平高于A549细胞。UGDH siRNA处理的LC-2 / ad细胞显示出明显降低的迁移和侵袭能力，但在UGDH siRNA处理的A549细胞中未观察到显着差异。这些数据表明，在肺AC患者中，除了不良的预后指标外，UGDH的表达和定位是早期的血清诊断指标。甚至在I期，在AC肺部AC患者中血清UGDH水平也特别高于健康对照组。在肺AC细胞系中，LC-2 / ad细胞中UGDH的核表达水平高于A549细胞。UGDH siRNA处理的LC-2 / ad细胞显示出明显降低的迁移和侵袭能力，但在UGDH siRNA处理的A549细胞中未观察到显着差异。这些数据表明，在肺AC患者中，除了不良的预后指标外，UGDH的表达和定位是早期的血清诊断指标。