Introduction: Capture of proteins and metabolites from Cultural Heritage (paintings, manuscripts, parchment etc.) has been done in the past via surface scraping and erasing, a method discouraged. The EVA (ethylene vinyl acetate) method consists of a plastic polymer in which strong cation and anion resins, admixed with C₈ and/or C₁₈, are embedded.

Areas covered: We review here the findings on different items stored in public libraries and archives: (a) the original manuscript of the novel Master y Margarita by Bulgakov; (b) the death registries of the lazaretto in the 1630 Milano plague; (c) the shirt worn by A. Chekhov in his death bed; (d) Kepler’s script on Hipparchus (in St. Petersburg National Archives); (e) the Memoirs of G. Casanova.

Expert opinion: The technique here surveyed appears to be a unique tool enabling exploration of any document stored in public archives, museum and private collections without damaging or contaminating the items under analysis. The amounts harvested from any surface are very minute, yet sufficient for analysis via advanced mass spectrometry instrumentation, thus permitting the identification of all captured material. It is hoped that the present review will stimulate the scientific community to adopt it for projects pertaining to Cultural Heritage.

简介：过去通过表面刮擦和擦除方法从文化遗产（绘画，手稿，羊皮纸等）中捕获蛋白质和代谢物，但不鼓励这样做。EVA（乙烯乙酸乙烯酯）方法由一种塑料聚合物组成，其中嵌入了与C ₈和/或C ₁₈混合的强阳离子和阴离子树脂。

涵盖领域：我们在这里回顾在公共图书馆和档案馆中存储的不同项目的发现：（a）布尔加科夫（Bulgakov）的小说《玛格丽塔大师》的原始手稿；（b）1630年米兰瘟疫中lazaretto的死亡登记簿；（c）契kh夫在死前所穿的衬衫；（d）开普勒关于希帕古斯的剧本（在圣彼得堡国家档案馆中）；（e）卡萨诺瓦回忆录。

专家意见：此处调查的技术似乎是一种独特的工具，可以对存储在公共档案馆，博物馆和私人收藏物中的任何文件进行浏览，而不会损坏或污染所分析的物品。从任何表面收获的数量都很少，但足以通过高级质谱仪进行分析，因此可以识别所有捕获的物质。希望本次审查会刺激科学界将其用于与文化遗产有关的项目。