Glass ceramics are widely used to enhance the functionality of inert metallic materials typically used for hard-tissue engineering. Biofunctionality of glass ceramics can in turn be significantly boosted with the addition of trace element dopants. Herein, we synthesized a nanostructured glass ceramic and used magnesium (Mg), which is known to promote osteoblast adhesion and proliferation, for further functionalization. The nanostructured akermanite glass ceramic (Ca2MgSi2O7) was used to coat Ti6Al4V substrates by the sol-gel method. Scanning and transmission electron microscopy as well as X-ray diffraction were used to assess the structural morphology and phase composition of the coating, respectively. The micrographs showed a uniform and crack-free coating structure. Atomic force microscopy observation revealed a disordered surface roughness for coated samples. In vitro cytocompatibility tests revealed that Saos-2 cells cultured on bare samples adopted a rounded morphology, whereas cells cultured on the coated samples represented a more spread out configuration and also increased proliferation. The characterizing tests confirmed the efficiency of the synthesis method and the in vitro biocompatibility of the synthesized coating, indicating its suitability to be used for bone implants.

中文翻译：

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用于骨科应用的 Ti6Al4V 纳米结构钙镁石微晶玻璃涂层

玻璃陶瓷被广泛用于增强通常用于硬组织工程的惰性金属材料的功能。微量元素掺杂剂的添加反过来可以显着提高玻璃陶瓷的生物功能性。在此，我们合成了一种纳米结构的玻璃陶瓷，并使用已知可促进成骨细胞粘附和增殖的镁 (Mg) 进行进一步功能化。纳米结构的钙镁石玻璃陶瓷 (Ca2MgSi2O7) 用于通过溶胶-凝胶法涂覆 Ti6Al4V 基材。扫描和透射电子显微镜以及 X 射线衍射分别用于评估涂层的结构形态和相组成。显微照片显示出均匀且无裂纹的涂层结构。原子力显微镜观察显示涂层样品的表面粗糙度无序。体外细胞相容性测试显示，在裸样品上培养的 Saos-2 细胞采用圆形形态，而在包被样品上培养的细胞表现出更分散的配置，并且增殖也增加。表征测试证实了合成方法的效率和合成涂层的体外生物相容性，表明其适用于骨植入物。