Abstract Microwave irradiation during tissue fixation and immunostaining reduces the sample preparation time. Microwave irradiation also facilitates the penetration of fixatives and antibody solutions into the tissues, resulting in efficient fixation and reduction of non-specific antibody binding, respectively. Experimental procedures involving immunofluorescence microscopy are time-consuming as this method relies on antigenantibody reaction. Here, we utilized a technique involving exposure of cultured cells and tissues to intermittent microwave irradiation and immunostaining of fixed samples. Intermittent microwave irradiation during fixation reduces the required incubation time with blocking and antibody solutions, and results in good preservation of the immunoreactivity of fixed cells. Microwave irradiation also reduces the non-specific binding of fluorescein-labeled antibodies. These microwave-assisted rapid immunofluorescence techniques are useful for analysis of molecular compositions in cultured cell systems.

中文翻译：

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使用微波辐射对培养细胞进行快速固定和免疫荧光染色

摘要 组织固定和免疫染色过程中的微波照射减少了样品制备时间。微波照射还有助于固定剂和抗体溶液渗透到组织中，分别导致有效固定和减少非特异性抗体结合。涉及免疫荧光显微镜的实验程序非常耗时，因为该方法依赖于抗原抗体反应。在这里，我们利用了一种技术，涉及将培养的细胞和组织暴露于间歇性微波辐射和固定样本的免疫染色。固定过程中的间歇性微波辐射减少了封闭液和抗体溶液所需的孵育时间，从而很好地保留了固定细胞的免疫反应性。微波辐射还降低了荧光素标记抗体的非特异性结合。这些微波辅助快速免疫荧光技术可用于分析培养细胞系统中的分子组成。