This review focuses on the methods and current trends in determination of neuraminidases (NAs) activity and evaluation of neuraminidase inhibitors (NAIs) by means of biochemical assays. These methods can be used, in principle, for any type of sialidase, with regard to substrate specificity and optimal conditions for enzymatic reaction. Considering the range of organisms producing sialidases, this review omits cell-based assays (plaque assays and study of cytopathic effect) and animal model studies, which are reviewed elsewhere concerning specific organisms. The present review also provides the brief introductory survey of role of sialic acids and neuraminidases, but main focus is on the methods of determining neuraminidase activity and evaluating neuraminidase inhibitors. The future prospect of improvement in analytical techniques regarding the enzymatic activity of sialidases is briefly outlined.

中文翻译：

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测定神经氨酸酶活性和评估神经氨酸酶抑制剂的方法和当前趋势。

这篇综述着重于确定神经氨酸酶（NAs）活性的方法和当前趋势，以及通过生化测定评估神经氨酸酶抑制剂（NAIs）的方法。就底物特异性和酶促反应的最佳条件而言，这些方法原则上可用于任何类型的唾液酸酶。考虑到产生唾液酸酶的生物的范围，该评价省略了基于细胞的测定（噬菌斑测定和细胞致病作用研究）和动物模型研究，这些研究在其他地方涉及特定生物。本综述还提供了唾液酸和神经氨酸酶作用的简要介绍，但主要重点是确定神经氨酸酶活性和评估神经氨酸酶抑制剂的方法。