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Estimated resistance of the malaria mosquito Anopheles messeae s.l. to the insecticide malathion.
Journal of Vector Ecology ( IF 1.7 ) Pub Date : 2019-05-23 , DOI: 10.1111/jvec.12328
Oleg V Vaulin 1 , Dmitry A Karagodin 1 , Elina M Baricheva 1 , Ilya K Zakharov 1
Affiliation  

Resistance to agricultural pesticides is an important and insufficiently studied concern for pest and disease vector research. We determined the malathion resistance of species in the Anopheles maculipennis mosquito group in a habitat near Novosibirsk, Russia. Most of the 851 individuals we measured were members of the Anopheles messeae s.l. complex (An. messeae and An. daciae species). The LC50 value for malathion was 0.052 mg/L for the mixed specimens, and we failed to find any differences between species. The LC50 value was within the range of values for malathion resistance of Anopheles stephensi and Culex quinquefasciatus. As the main resistance mechanism to organophosphate and carbamate insecticides is a single mononucleotide substitution in the ace‐1 gene, we searched for this mutation in An. messeae s.l. and An. beklemishevi by restriction analysis. This mutation was not found in 347 of the specimens. We sequenced the ace‐1 gene fragment for 24 specimens from four species of the Anopheles maculipennis group, including An. messeae, An. daciae, An. atroparvus, and An. beklemishevi. These specimens harbored a nucleotide substitution in the triplet where a mutation can lead to insecticide resistance, but this substitution would make it difficult for the resistance to develop. Since the studied specimens belong to branches of the Palearctic portion of the Anopheles maculipennis group, we suspect that all other Palearctic species of this group would have difficulties harboring the ace‐1 mutation that would lead to organophosphate and carbamate resistance.

中文翻译:

疟疾蚊子按蚊sl对杀虫剂马拉硫磷的估计抗性。

对农业杀虫剂的抗性是病虫害媒介研究的重要且研究不足的问题。我们在俄罗斯新西伯利亚附近的一个栖息地中确定了按蚊大群按蚊的马拉硫磷抗性。我们测得的851个人中,大多数是信使按蚊An。按蚊按蚊An。daciae)种)的成员。混合标本中马拉硫磷的LC 50值为0.052 mg / L,我们没有发现物种之间的任何差异。LC 50值在斯蒂芬按蚊库蚊(Culex quinquefasciatus)的马拉硫磷抗性值范围内。由于对有机磷酸酯和氨基甲酸酯类杀虫剂的主要抗性机制是ace-1基因中的单个单核苷酸取代,因此我们在An中搜索了此突变messeae sl和An。通过限制分析对beklemishevi进行分析。在347个样本中未发现此突变。我们对ace-1基因片段进行了测序,该片段来自包括An。在内四种按蚊Maculipennis组的24个标本。信使,安。达契亚 atroparvusAn。别克利米谢维。这些标本在三联体中具有核苷酸取代,其中突变可导致对杀虫剂的抗性,但是这种取代将使抗性难以发展。由于所研究的标本属于按蚊按蚊群的古菌科部分的分支,因此我们怀疑该组中的所有其他古菌种都难以携带ace-1突变,从而导致有机磷酸酯和氨基甲酸酯抗性。
更新日期:2019-05-23
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