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Cells on the move: Modulation of guidance cues during germ cell migration.
FLY ( IF 1.2 ) Pub Date : 2017-03-31 , DOI: 10.1080/19336934.2017.1304332
Girish Deshpande 1 , Justinn Barr 1 , Offer Gerlitz 2 , Lyubov Lebedeva 3 , Yulii Shidlovskii 3 , Paul Schedl 1, 3
Affiliation  

In Drosophila melanogaster the progenitors of the germ-line stem cells, the primordial germ cells (PGCs) are formed on the outside surface of the early embryo, while the somatic gonadal precursor cells (SGPs) are specified during mid-embryogenesis. To form the primitive embryonic gonad, the PGCs travel from outside of the embryo, across the mid-gut and then migrate through the mesoderm to the SGPs. The migratory path of PGCs is dictated by a series of attractive and repulsive cues. Studies in our laboratory have shown that one of the key chemoattractants is the Hedgehog (Hh) ligand. Although, Hh is expressed in other cell types, the long-distance transmission of this ligand is specifically potentiated in the SGPs by the hmgcr isoprenoid biosynthetic pathway. The distant transmission of the Hh ligand is gated by restricting expression of hmgcr to the SGPs. This is particularly relevant in light of the recent findings that an ABC transporter, mdr49 also acts in a mesoderm specific manner to release the germ cell attractant. Our studies have demonstrated that mdr49 functions in hh signaling likely via its role in the transport of cholesterol. Given the importance of cholesterol in the processing and long distance transmission of the Hh ligand, this observation has opened up an exciting avenue concerning the possible role of components of the sterol transport machinery in PGC migration.



中文翻译:

移动中的细胞:生殖细胞迁移过程中指导信号的调节。

果蝇中,生殖系干细胞的祖细胞,原始生殖细胞(PGC)在早期胚胎的外表面上形成,而体细胞性腺前体细胞(SGP)在胚胎中期被指定。为了形成原始的胚胎性腺,PGC从胚胎的外部穿过中肠,然后通过中胚层迁移到SGP。PGC的迁移路径由一系列吸引人和排斥的线索所决定。我们实验室的研究表明,主要的化学吸引剂之一是刺猬(Hh)配体。尽管Hh在其他细胞类型中表达,但hmgcr在SGP中特异性增强了该配体的长距离传播类异戊二烯的生物合成途径。Hh配体的远距离传输通过限制hmgcr的表达限制在SGP上。鉴于最近的发现,这特别相关,即ABC转运蛋白mdr49也以中胚层特异性方式起作用以释放生殖细胞引诱剂。我们的研究表明,mdr49可能通过其在胆固醇运输中的作用而在hh信号中起作用。考虑到胆固醇在Hh配体的加工和远距离传输中的重要性,这一发现开辟了令人激动的途径,涉及固醇转运机制的组分在PGC迁移中的可能作用。

更新日期:2017-03-31
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