Bone marrow and adipose tissue human mesenchymal stem cells were seeded in highly performing 3D gelatin-chitosan hybrid hydrogels of varying chitosan content in the presence of human platelet lysate and evaluated for their proliferation and osteogenic differentiation. Both bone marrow and adipose tissue human mesenchymal stem cells in gelatin-chitosan hybrid hydrogel 1 (chitosan content 8.1%) or gelatin-chitosan hybrid hydrogel 2 (chitosan 14.9%) showed high levels of viability (80%-90%), and their proliferation and osteogenic differentiation was significantly higher with human platelet lysate compared to fetal bovine serum, particularly in gelatin-chitosan hybrid hydrogel 1. Mineralization was detected early, after 21 days of culture, when human platelet lysate was used in the presence of osteogenic stimuli. Proteomic characterization of human platelet lysate highlighted 59 proteins mainly involved in functions related to cell adhesion, cellular repairing mechanisms, and regulation of cell differentiation. In conclusion, the combination of our gelatin-chitosan hybrid hydrogels with hPL represents a promising strategy for bone regenerative medicine using human mesenchymal stem cells.

中文翻译：

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3D明胶-壳聚糖杂化水凝胶与人血小板溶解产物结合可高度支持人间充质干细胞增殖和成骨分化。

在人血小板裂解物存在的情况下，将骨髓和脂肪组织的人间充质干细胞接种在不同壳聚糖含量的高性能3D明胶-壳聚糖混合水凝胶中，并评估其增殖和成骨分化。明胶-壳聚糖混合水凝胶1（壳聚糖含量8.1％）或明胶-壳聚糖混合水凝胶2（壳聚糖14.9％）中的骨髓和脂肪组织人间充质干细胞均显示出高水平的活力（80％-90％），与胎儿牛血清相比，人血小板裂解物的增殖和成骨分化明显更高，尤其是在明胶-壳聚糖杂化水凝胶1中。培养21天后，在存在成骨刺激物的情况下使用人血小板裂解物时，早期发现了矿化作用。人血小板裂解物的蛋白质组学表征突出显示了59种蛋白质，主要参与与细胞粘附，细胞修复机制和细胞分化调控有关的功能。总之，我们的明胶-壳聚糖混合水凝胶与hPL的组合代表了使用人间充质干细胞进行骨再生医学的有前途的策略。