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β-Hemolytic Streptococcus anginosus subsp. anginosus causes streptolysin S-dependent cytotoxicity to human cell culture lines in vitro.
Journal of Oral Microbiology ( IF 4.5 ) Pub Date : 2019-05-08 , DOI: 10.1080/20002297.2019.1609839
Atsushi Tabata 1, 2 , Takuya Yamada 2 , Hiromi Ohtani 2 , Kazuto Ohkura 3 , Toshifumi Tomoyasu 1, 2 , Hideaki Nagamune 1, 2
Affiliation  

Background: Streptococcus anginosus subsp. anginosus (SAA) is one of the opportunistic pathogens in humans that inhabits the oral cavity. The type strain of SAA, NCTC10713T, showed clear β-hemolysis on blood agar plates, and the sole β-hemolytic factor revealed two streptolysin S (SLS) molecules. SLS is well known as the peptide hemolysin produced from the human pathogen S. pyogenes and shows not only hemolytic activity on erythrocytes but also cytotoxic activity in cell culture lines in vitro and in vivo, such as in a mouse infection model. However, no cytotoxic activity of SLS produced from β-hemolytic SAA (β-SAA) has been reported so far. Objective and Design: In this study, the SLS-dependent cytotoxicity of the β-SAA strains including the genetically modified strains was investigated in vitro. Results: The SLS-producing β-SAA showed cytotoxicity in human cell culture lines under the co-cultivation condition and it was found that this cytotoxicity was caused by the SLS secreted into the extracellular milieu. Conclusion: The results from this study suggest that the SLS produced from β-SAA might indicate the cytotoxic potential similar to that of the SLS from S. pyogenes and the SLS-producing β-SAA would be recognized as “a wolf in sheep’s clothing” More attention will be paid to the pathogenicity of β-hemolytic Anginosus group streptococci.



中文翻译:

β-溶血性链球菌Anginosus亚种。心绞痛在体外对人细胞培养株产生链球菌溶血素S依赖性细胞毒性。

背景: 链球菌(Anginosus)亚种。冠心病(SAA)是人类居住在口腔中的机会病原体之一。SAA的型菌株NCTC10713 T在琼脂平板上显示出清晰的β-溶血,唯一的β-溶血因子显示了两个链球菌溶血素S(SLS)分子。SLS众所周知是从人类病原体化脓性链球菌产生的肽溶血素,不仅在体外体内(例如在小鼠感染模型中)在细胞培养物中显示出对红细胞的溶血活性,而且还显示出细胞毒活性。然而,迄今为止,尚未报道由β-溶血性SAA(β-SAA)产生的SLS的细胞毒活性。目标与设计:在这项研究中,对包括转基因菌株在内的β-SAA菌株的SLS依赖性细胞毒性进行了体外研究结果:在共培养条件下,产生SLS的β-SAA在人细胞培养物中显示出细胞毒性,并且发现这种细胞毒性是由分泌到细胞外环境中的SLS引起的。结论:这项研究的结果表明,β-SAA产生的SLS可能表明与化脓性链球菌产生的SLS具有相似的细胞毒性潜能,而产生SLS的β-SAA被认为是“披着羊皮的狼”。 β-溶血性Anginosus组链球菌的致病性将得到更多关注。

更新日期:2019-05-08
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