ABSTRACT DNA transduction across aqueous solutions has been reported previously. In this study, we examined a few key factors affecting DNA transduction rate in an extremely low frequency electromagnetic field. These include: the chemical composition of the aqueous solutions, the type of experimental vessel, the dilution step, and the origin of the DNA fragments. The results indicate that partially introducing essential ingredients for DNA amplification (i.e. dNTPs and PCR buffer) to the aqueous solution enhanced the transduction rate greatly, and transduction vessels made of hydrophilic quartz yielded more favorable results than vessels made of hydrophobic plastic. In addition, performing a serial dilution to the transduction solution more than doubled the transduction rate compared to that without the dilution step. For the DNA fragments used in this study, there was one with a pathogenic origin and two with non-pathogenic origins. However, all three fragments achieved DNA transduction regardless of the difference in their origins. The experimental setup for eliminating the false positives caused by both biological and potentially physical contamination is also described.

中文翻译：

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弱电磁场诱导DNA转导的限速因素

摘要 先前已报道过水溶液中的 DNA 转导。在这项研究中，我们研究了在极低频电磁场中影响 DNA 转导率的几个关键因素。这些包括：水溶液的化学成分、实验容器的类型、稀释步骤和 DNA 片段的来源。结果表明，将DNA扩增的必要成分（即dNTPs和PCR缓冲液）部分引入水溶液中可大大提高转导率，亲水石英制成的转导容器比疏水塑料制成的转导容器产生更有利的结果。此外，与没有稀释步骤的情况相比，对转导溶液进行连续稀释可使转导率提高一倍以上。对于本研究中使用的 DNA 片段，有一个具有致病性来源，两个具有非致病性来源。然而，无论来源不同，所有三个片段都实现了 DNA 转导。还描述了用于消除由生物和潜在物理污染引起的误报的实验设置。