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Taxonomy and toxicity of Prorocentrum from Perhentian Islands (Malaysia), with a description of a non-toxigenic species Prorocentrum malayense sp. nov. (Dinophyceae).
Harmful Algae ( IF 6.6 ) Pub Date : 2019-03-15 , DOI: 10.1016/j.hal.2019.01.007
Zhen Fei Lim 1 , Zhaohe Luo 2 , Li Keat Lee 1 , Kieng Soon Hii 1 , Sing Tung Teng 3 , Leo Lai Chan 4 , Nicolas Chomérat 5 , Bernd Krock 6 , Haifeng Gu 2 , Po Teen Lim 1 , Chui Pin Leaw 1
Affiliation  

Thirteen isolates of Prorocentrum species were established from the coral reefs of Perhentian Islands Marine Park, Malaysia and underwent morphological observations and molecular characterization. Six species were found: P. caipirignum, P. concavum, P. cf. emarginatum, P. lima, P. mexicanum and a new morphotype, herein designated as P. malayense sp. nov. Prorocentrum malayense, a species closely related to P. leve, P. cf. foraminosum, P. sp. aff. foraminossum, and P. concavum (Clade A sensu Chomérat et al. 2018), is distinguished from its congeners as having larger thecal pore size and a more deeply excavated V-shaped periflagellar area. Platelet arrangement in the periflagellar area of P. malayense is unique, with the presence of platelet 1a and 1b, platelet 2 being the most anterior platelet, and a broad calabash-shaped platelet 3. The species exhibits consistent genetic sequence divergences for the nuclear-encoded large subunit ribosomal RNA gene (LSU rDNA) and the second internal transcribed spacer (ITS2). The phylogenetic inferences further confirmed that it represents an independent lineage, closely related to species in Clade A sensu Chomérat et al. Pairwise comparison of ITS2 transcripts with its closest relatives revealed the presence of compensatory base changes (CBCs). Toxicity analysis showed detectable levels of okadaic acid in P. lima (1.0–1.6 pg cell˗1) and P. caipirignum (3.1 pg cell˗1); this is the first report of toxigenic P. caipirignum in the Southeast Asian region. Other Prorocentrum species tested, including the new species, however, were below the detection limit.



中文翻译:

来自Perhentian群岛(马来西亚)的原肠原虫的分类学和毒性,并描述了非产毒物种Prorocentrum malayense sp.。十一月 (恐龙科)。

从马来西亚Perhentian群岛海洋公园的珊瑚礁中建立了13种原中心菌的分离株,并对其进行了形态学观察和分子表征。发现六种:PcaipirignumPconcavumP。cf. emarginatumP利马P墨西哥和一种新的形态型,在此称为马来酸假单胞菌。十一月 马来原螺,与P密切相关的物种。LEVEP。cf.foraminosumP。sp。aff。foraminossum,和Pconcavum(进化枝甲意义上Chomérat等人2018),从它的同源区分为具有较大卵泡膜孔径和更深入地挖掘V形periflagellar区域。P鞭毛周围区的血小板排列。马来西亚独特,有血小板1a和1b,血小板2是最前面的血小板,以及宽阔的葫芦形血小板3。该物种对核编码大亚基核糖体RNA基因(LSU rDNA)表现出一致的遗传序列差异。第二内部转录间隔物(ITS2)。系统发育推理进一步证实,它代表一个独立的谱系,密切相关的进化枝甲物种意义上Chomérat等。ITS2成绩单与其最接近的亲戚的成对比较显示,存在代偿性碱基变化(CBC)。毒性分析显示在冈田酸的可检测水平的P利马1.0–1.6 pg细胞˗1)和开胃菜(3.1 pg细胞˗1); 这是有毒的P的首次报道。东南亚地区的caipirignum。但是,测试的其他原肠种,包括新种,都低于检出限。

更新日期:2019-03-15
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