Recent concern about the possible secondary spread of vCJD through blood transfusion and blood products has highlighted the need for a sensitive test for the identification of PrP(TSE/res) in clinical specimens collected in a non-invasive way. In addition, a more accurate estimate of the prevalence of pre-clinical vCJD in the population may be possible if there were a test that could be applied to easily available material such as urine. As a step towards this goal,the detection of putative PrP(TSE/res) in the urine of CJD patients has been improved, based on Proteinase K digestion of samples and western blotting. The modified western blot uses concentrated urine as a starting material. After proteolytic treatment followed by electrophoresis and western blotting, membranes are incubated with an anti-PrP antibody conjugated directly with horseradish peroxidase. This study was conducted on urine samples of CJD and other neurodegenerative disease affected individuals. Proteinase K resistant high molecular weight proteins were detected, which are suggested to be a complex of urinary PrP and immunoglobulin proteins. Whether urine can be used as a diagnostic tool for the detection of PrP could not be answered in this study.

中文翻译：

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检测克雅氏病和其他神经退行性疾病患者尿液中蛋白酶 K 抗性蛋白。

最近对 vCJD 可能通过输血和血液制品进行二次传播的担忧突出了对以非侵入性方式收集的临床标本中鉴定 PrP(TSE/res) 的敏感测试的必要性。此外，如果有一种可应用于容易获得的材料（如尿液）的测试，则可能更准确地估计人群中临床前 vCJD 的流行率。作为朝着这一目标迈出的一步，基于蛋白酶 K 消化样品和蛋白质印迹，改进了 CJD 患者尿液中推定 PrP(TSE/res) 的检测。改良的蛋白质印迹使用浓缩的尿液作为起始材料。经过蛋白水解处理，然后进行电泳和蛋白质印迹，膜与直接与辣根过氧化物酶偶联的抗 PrP 抗体一起孵育。这项研究是对克雅氏病和其他神经退行性疾病患者的尿液样本进行的。检测到蛋白酶 K 抗性高分子量蛋白，提示为尿 PrP 和免疫球蛋白的复合物。本研究无法回答尿液是否可以作为检测PrP的诊断工具。