RNAs are exported from the nucleus to the cytoplasm, where they undergo translation and produce proteins needed for the cellular life cycle. Some mRNAs are targeted by different RNA decay mechanisms and thereby undergo degradation. The 5'-->3' degradation machinery localizes to cytoplasmic complexes termed P bodies (PBs). They function in RNA turnover, translational repression, RNA-mediated silencing, and RNA storage. A quantitative live-cell imaging approach to study the dynamic aspects of PB trafficking in the cytoplasm revealed that PB movements are rather confined and dependent on an existing microtubule network. Microtubule depolymerization led to a drastic decrease in PB mobility, as well as a release of regulation on PB assembly and a dramatic increase in PB numbers. The different aspects of PB trafficking and encounters with mRNA molecules in the cytoplasm are discussed.

中文翻译：

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P 体的细胞内运输和动力学。

RNA 从细胞核输出到细胞质，在那里它们进行翻译并产生细胞生命周期所需的蛋白质。一些 mRNA 被不同的 RNA 衰变机制靶向，从而发生降解。5'-->3' 降解机制定位于称为 P 体 (PBs) 的细胞质复合物。它们在 RNA 转换、翻译抑制、RNA 介导的沉默和 RNA 储存中起作用。一种用于研究细胞质中 PB 运输动态方面的定量活细胞成像方法表明，PB 运动相当受限并且依赖于现有的微管网络。微管解聚导致 PB 迁移率急剧下降，以及对 PB 组装的监管释放和 PB 数量的急剧增加。