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Anti-LRP/LR antibody W3 hampers peripheral PrPSc propagation in scrapie infected mice.
Prion ( IF 2.3 ) Pub Date : 2007-07-07 , DOI: 10.4161/pri.1.3.5273
Chantal Zuber 1 , Gerda Mitteregger , Claudia Pace , Inga Zerr , Hans A Kretzschmar , Stefan Weiss
Affiliation  

We identified the 37kDa/67kDa laminin receptor (LRP/LR) as a cell surface receptor for the cellular prion protein (PrP(c)) and the infectious prion protein (PrP(Sc)). Recently, we showed that anti-LRP/LR antibody W3 cured scrapie infected N2a cells. Here, we demonstrate that W3 delivered by passive immunotransfer into C57BL/6 mice reduced the PrP(Sc) content in the spleen significantly by 66%, demonstrating an impairment of the peripheral PrP(Sc) propagation. In addition, we observed a 1.8-fold increase in survival of anti-LRP/LR antibody W3 treated mice (mean survival of 31 days) compared to preimmune serum treated control animals (mean survival of 17 days). We conclude that the significant effect of anti-LRP/LR antibody W3 on the reduction of peripheral PrP(Sc) propagation might be due to the blockage of the prion receptor LRP/LR which is required, as previously shown in vitro, for PrP(Sc) propagation in vivo.

中文翻译:

抗 LRP/LR 抗体 W3 阻碍了痒病感染小鼠的外周 PrPSc 传播。

我们将 37kDa/67kDa 层粘连蛋白受体 (LRP/LR) 确定为细胞朊病毒蛋白 (PrP(c)) 和传染性朊病毒蛋白 (PrP(Sc)) 的细胞表面受体。最近,我们发现抗 LRP/LR 抗体 W3 治愈了感染痒病的 N2a 细胞。在这里,我们证明通过被动免疫转移传递到 C57BL/6 小鼠的 W3 将脾脏中的 PrP(Sc) 含量显着降低了 66%,表明外周 PrP(Sc) 传播受损。此外,我们观察到与免疫前血清处理的对照动物(平均存活 17 天)相比,抗 LRP/LR 抗体 W3 处理的小鼠的存活率(平均存活 31 天)增加了 1.8 倍。
更新日期:2019-11-01
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