BACKGROUND Von Willebrand factor (VWF) has a central role in primary haemostasis and is a popular pharmaceutical target in the prevention and treatment of disorders such as acute coronary syndrome and thrombotic thrombocytopenic purpura. We have evaluated numerous possible treatments targeting VWF in the chacma baboon. Unfortunately, no data exist regarding the molecular similarity of the chacma baboon and human VWF protein, resulting in limited translatability of results. METHODS Sanger sequencing was performed of the functionally vital VWF exon 28. The sequences were then compared to the human reference sequence. RESULTS The baboon and human VWF amino acid sequences were 99.1% similar, with only 4 radical amino acid changes found. No radical amino acid changes were found within the functionally vital areas of the amino acid sequence. CONCLUSION The chacma baboon VWF is similar enough to the human to produce reliable and translatable pre-clinical results with human-targeted anti-VWF agents.

中文翻译：

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Chacma狒狒的分子适用性在以人为目标的冯·威勒布兰德因子定向研究中。

背景技术Von Willebrand因子（VWF）在原发性止血中起着核心作用，并且在预防和治疗诸如急性冠状动脉综合征和血栓性血小板减少性紫癜等疾病中是受欢迎的药物靶标。我们评估了在狒狒中针对VWF的多种可能的治疗方法。不幸的是，尚无关于狒狒和人VWF蛋白的分子相似性的数据，导致结果的可翻译性有限。方法对功能至关重要的VWF外显子28进行Sanger测序。然后将该序列与人类参考序列进行比较。结果狒狒和人VWF氨基酸序列相似度为99.1％，仅发现4个自由基氨基酸变化。在氨基酸序列的功能关键区域内未发现自由基氨基酸变化。