Combining the surface modification and molecular imprinting technique, a novel piezoelectric sensing platform with excellent molecular recognition capability was established for the detection of uric acid (UA) based on the immobilization of TiO2 nanoparticles onto quartz crystal microbalance (QCM) electrode and modification of molecularly imprinted TiO2 (MIT) layer on TiO2 nanoparticles. The performance of the fabricated biosensor was evaluated, and the results indicated that the biosensor exhibited high sensitivity in UA detection, with a linear range from 0.04 to 45 μM and a limit of detection of 0.01 μM. Moreover, the biosensor presented high selectivity towards UA in comparison with other interferents. The analytical application of the UA biosensor confirmed the feasibility of UA detection in urine sample.

中文翻译：

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用分子印迹的TiO2修饰纳米晶TiO2涂层以识别尿酸。

结合表面修饰和分子印迹技术，基于TiO2纳米粒子固定在石英晶体微天平（QCM）电极上，并对分子印迹进行改性，建立了具有优异分子识别能力的新型压电传感平台，用于尿酸（UA）的检测。 TiO2纳米颗粒上的TiO2（MIT）层。评价了所制造的生物传感器的性能，结果表明该生物传感器在UA检测中表现出高灵敏度，线性范围为0.04至45μM，检出限为0.01μM。此外，与其他干扰物相比，生物传感器对UA的选择性高。UA生物传感器的分析应用证实了尿液样品中UA检测的可行性。