This study aimed to investigate the protective effects of two citrus flavonoids, hesperidin and naringin, on alcohol-induced thigmotaxis behavioural and developmental defects in zebrafish. The results of behavioural assays indicated that acute exposure to 1% alcohol could induce thigmotaxis behaviour in zebrafish larvae at 3 and 5 days post-fertilization (dpf), whereas hesperidin and naringin could inhibit the thigmotaxis behaviour in zebrafish larvae. Moreover, developmental administration of hesperidin and naringin reduced the levels of morphological defects and apoptosis induced by developmental alcohol exposure. In conclusion, this study suggests that the citrus flavonoids hesperidin and naringin could reduce the effects of alcohol damage on embryonic development and neurobehaviour in zebrafish larvae, which would provide useful knowledge for assessing the underlying mechanism of citrus flavonoids and their protective effects.

中文翻译：

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柑橘类黄酮橙皮苷和柚皮苷减轻了酒精引起的斑马鱼幼虫的行为改变和发育缺陷。

这项研究旨在调查两种柑桔类黄酮，橙皮苷和柚皮苷对酒精诱导的斑马鱼触变性行为和发育缺陷的保护作用。行为分析的结果表明，在受精后（dpf）的第3天和第5天，急性暴露于1％的酒精可引起斑马鱼幼虫的趋血性行为，而橙皮苷和柚皮苷可抑制斑马鱼幼虫的趋血性行为。此外，橙皮苷和柚皮苷的发育性给药减少了发育性酒精暴露诱导的形态学缺陷和凋亡水平。总之，这项研究表明，柑桔类黄酮橙皮苷和柚皮苷可以减少酒精对斑马鱼幼虫胚胎发育和神经行为的影响，