Adult Cochliomyia hominivorax (Coquerel), treated with graded doses of N,N '-tetramethylenebis(1-aziridinecarboxamide), were mated with untreated flies, and the frequency of induced dominant lethal mutations was determined. At first, the percentages of mutations increased rapidly with increasing dose, then the percentages increased more slowly. Intrathoracically injected flies required about 1/20 the topical dose needed to achieve an equal percentage of induced mutations in the mature sperm of treated flies. The sensitivity of oocytes to the chemical mutagen increased as the meiotic stage of the nuclei advanced: 3-day-old oocytes and mature sperm were about equally susceptible; 6- and 0-day-old oocytes were more or less susceptible, respectively, than the 3-day-old oocytes.

中文翻译：

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N,N'-Tetraniethylenebis (1-Aziridinecarboxamide) 在螺旋虫蝇的精子和卵细胞中诱导的显性致死突变的剂量反应曲线

成年 Cochliomyia hominivorax (Coquerel)，用分级剂量的 N，N'-四亚甲基双 (1-氮丙啶甲酰胺) 处理，与未经处理的苍蝇交配，并确定了诱导显性致死突变的频率。起初，突变的百分比随着剂量的增加而迅速增加，然后百分比增加得更慢。胸腔内注射的果蝇需要大约 1/20 的局部剂量，才能在处理过的果蝇的成熟精子中实现相同百分比的诱导突变。随着细胞核减数分裂阶段的进展，卵母细胞对化学诱变剂的敏感性增加：3 天大的卵母细胞和成熟的精子大致相同；6 天和 0 天大的卵母细胞分别比 3 天大的卵母细胞或多或少易感。