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Evaluation of Formalin Fixation for Tissue Biopsies Using Shear Wave Laser Speckle Imaging System
IEEE Journal of Translational Engineering in Health and Medicine ( IF 3.4 ) Pub Date : 2019-01-01 , DOI: 10.1109/jtehm.2019.2909914
Saniel D Lim 1, 2, 3 , Qixuan Huang 2, 4 , Eric J Seibel 1, 2, 3
Affiliation  

Chemical fixation is the slowest and often the most uncontrolled step in the multi-step process of preparing tissue for histopathology. In order to reduce the time from taking a core needle biopsy to making a diagnosis, a new approach is proposed that optically monitors the common formalin fixation process. A low-cost and highly-sensitive laser speckle imaging technique is developed to measure shear wave velocity in a biospecimen as small as 0.5 mm in thickness submerged in millifluidic channels. Shear wave velocity, which is the indicator of tissue mechanical property and induced by piezoelectric-actuation, was monitored using gelatin phantom and chicken breast during fixation, as well as post-fixed liver and colon tissues from human. Fixation levels in terms of shear wave velocity increased by approximately 271.0% and 130.8% in gelatin phantom and chicken breast, respectively, before reaching the plateaus at 10.91 m/s and 7.88 m/s. Within these small specimens, the plateaus levels and times varied with location of measurement, and between gelatin and chicken breast. This optical-based approach demonstrates the feasibility of fine-tuning preanalytical variables, such as fixation time, for a rapid and accurate histopathological evaluation; provides a quality metric during the tissue preparation protocol performed in most pathology labs; and introduces the millifluidic chamber that can be engineered to be a future disposable device that automates biopsy processing and imaging.

中文翻译:

使用剪切波激光散斑成像系统评估福尔马林固定组织活检

在为组织病理学准备组织的多步骤过程中,化学固定是最慢且通常最不受控制的步骤。为了减少从进行空芯针活检到做出诊断的时间,提出了一种新方法,可以对常见的福尔马林固定过程进行光学监测。开发了一种低成本和高灵敏度的激光散斑成像技术,用于测量浸没在微流体通道中的厚度小至 0.5 毫米的生物样本中的剪切波速度。剪切波速度是组织力学性能的指标,由压电驱动引起,在固定过程中使用明胶体模和鸡胸肉以及固定后的人体肝脏和结肠组织进行监测。剪切波速度方面的固定水平增加了大约 271.0% 和 130。在以 10.91 m/s 和 7.88 m/s 到达高原之前,在明胶幻影和鸡胸肉中分别为 8%。在这些小样本中,高原水平和时间随测量位置以及明胶和鸡胸肉之间的变化而变化。这种基于光学的方法证明了微调分析前变量(如固定时间)的可行性,以进行快速准确的组织病理学评估;在大多数病理实验室执行的组织制备协议期间提供质量指标;并介绍了微流体室,该室可以设计为未来的一次性设备,可自动进行活检处理和成像。以及明胶和鸡胸肉之间。这种基于光学的方法证明了微调分析前变量(如固定时间)的可行性,以进行快速准确的组织病理学评估;在大多数病理实验室执行的组织制备协议期间提供质量指标;并介绍了微流体室,该室可以设计为未来的一次性设备,可自动进行活检处理和成像。以及明胶和鸡胸肉之间。这种基于光学的方法证明了微调分析前变量(如固定时间)的可行性,以进行快速准确的组织病理学评估;在大多数病理实验室执行的组织制备协议期间提供质量指标;并介绍了微流体室,该室可以设计为未来的一次性设备,可自动进行活检处理和成像。
更新日期:2019-01-01
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