Netrin-1 is a potent axonal and neuronal guidance cue in the developing nervous system. Netrin-1 functions are mediated by its receptors, such as deleted in colorectal cancer (DCC) present on axons and neurons. Localization of DCC and Netrin-1 on various types of enteric neurons and their role in the mature enteric nervous system is unknown. The results of our study revealed that almost all enteric neurons and processes express DCC and Netrin-1 in the adult mice. Netrin-1-like-immunoreactivity (IR) was detected in the cytoplasm of neurons with some showing strong or weak staining. The majority of Netrin-1-like-immunoreactive enteric neurons were choline acetyltransferase (ChAT)-positive. However, ~19% of neurons were strongly Netrin-1-like-positive but ChAT-negative while ~8% of neurons were Netrin-1-like-negative but strongly ChAT-positive. In contrast, almost all nitric oxide synthase (nNOS)-positive enteric neurons displayed strong Netrin-1-like-IR. This differential intensity of Netrin-1 expression in the myenteric neurons might determine major neuronal subtypes regulating intestinal motility, ChAT-IR excitatory, and nNOS-IR inhibitory muscle motor and interneurons. This is the first study demonstrating the localization of DCC and Netrin-1 in the colonic myenteric plexus of the adult mice and their expression level determining two major neuronal subtypes regulating intestinal motility.

中文翻译：

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Netrin-1样免疫反应性与DCC共表达，并且在成年小鼠结肠的肠系膜胆碱能和亚硝能神经元中具有差异水平。

Netrin-1是发育中的神经系统中有效的轴突和神经元指导信号。Netrin-1的功能由其受体介导，例如在轴突和神经元上存在的结直肠癌（DCC）中缺失。尚不清楚DCC和Netrin-1在各种类型的肠神经元上的定位及其在成熟肠神经系统中的作用。我们的研究结果表明，成年小鼠中几乎所有的肠神经元和过程都表达DCC和Netrin-1。在神经元细胞质中检测到了Netrin-1样免疫反应性（IR），其中有些显示出强染色或弱染色。大部分的Netrin-1样免疫反应性肠神经元是胆碱乙酰转移酶（ChAT）阳性的。但是，约19％的神经元呈Netrin-1样阳性但ChAT阴性，而约8％的神经元呈Netrin-1样阴性但ChAT阳性。相比之下，几乎所有一氧化氮合酶（nNOS）阳性的肠神经元都表现出强的Netrin-1样IR。肌网神经元中Netrin-1表达的这种差异强度可能决定了调节肠蠕动，ChAT-IR兴奋性和nNOS-IR抑制性肌肉运动和中间神经元的主要神经元亚型。这是第一项证明DCC和Netrin-1在成年小鼠结肠肌层神经丛中的定位及其表达水平的研究，确定了两种主要的调节肠蠕动的神经元亚型。以及nNOS-IR抑制肌肉运动和中间神经元。这是第一项证明DCC和Netrin-1在成年小鼠结肠肌层神经丛中的定位及其表达水平的研究，确定了两种主要的调节肠蠕动的神经元亚型。以及nNOS-IR抑制肌肉运动和中间神经元。这是第一项证明DCC和Netrin-1在成年小鼠结肠肌层神经丛中的定位及其表达水平的研究，确定了两种主要的调节肠蠕动的神经元亚型。