Alternaria species have been reported to infect a wide variety of vegetables, fruits, and cereal crops. Wheat is one of the most consumed cereal worldwide. A sensitive HPLC-DAD methodology was applied to quantify alternariol (AOH), alternariol methyl ether (AME) and tenuazonic acid (TeA) in 65 samples of whole wheat, bran, and flour. The extraction methodology allowed extracting the three toxins simultaneously. Limits of detection in wheat were 3.4, 4.5, and 0.5 µg kg⁻¹ for AOH, AME and TeA, respectively. For bran, these data were 3.1, 4.5, and 12 µg kg⁻¹ and for flour 50, 70, and 14 µg kg⁻¹, respectively. The studied recoveries were higher than 70% and RSD was below 10%. Wheat and bran samples showed low AOH and AME contamination compared to TeA. The averages levels found for TeA in wheat, bran and flour were 19,190, 16,760, and 7360 µg kg⁻¹, respectively.

据报道，交链孢菌种感染多种蔬菜，水果和谷类作物。小麦是世界上最消耗的谷物之一。应用灵敏的HPLC-DAD方法对65个全麦，麸皮和面粉样品中的交替糖（AOH），交替糖甲醚（AME）和Tenuazonic酸（TeA）进行定量。提取方法允许同时提取三种毒素。小麦中AOH，AME和TeA的检出限分别为3.4、4.5和0.5 µg kg ^-1。对于麸皮，这些数据为3.1、4.5和12 µg kg ^-1，对于面粉50、70和14 µg kg ^-1， 分别。研究的回收率高于70％，RSD低于10％。与TeA相比，小麦和麸皮样品显示出较低的AOH和AME污染。小麦，麸皮和面粉中TeA的平均含量分别为19,190、16,760和7360 µg kg ^-1。