With a diverse physiological interface to colonize, mammalian skin is the first line of defense against pathogen invasion and harbors a consortium of microbes integral in maintenance of epithelial barrier function and disease prevention. While the dynamic roles of skin bacterial residents are expansively studied, contributions of fungal constituents, the mycobiome, are largely overlooked. As a result, their influence during skin injury, such as disruption of skin integrity in burn injury and impairment of host immune defense system, is not clearly delineated. Burn patients experience a high risk of developing hard-to-treat fungal infections in comparison to other hospitalized patients. To discern the changes in the mycobiome profile and network assembly during cutaneous burn-injury, a rat scald burn model was used to survey the mycobiome in healthy (n = 30) (sham-burned) and burned (n = 24) skin over an 11-day period. The healthy skin demonstrated inter-animal heterogeneity over time, while the burned skin mycobiome transitioned toward a temporally stabile community with declining inter-animal variation starting at day 3 post-burn injury. Driven primarily by a significant increase in relative abundance of Candida, fungal species richness and abundance of the burned skin decreased, especially in days 7 and 11 post-burn. The network architecture of rat skin mycobiome displayed community reorganization toward increased network fragility and decreased stability compared to the healthy rat skin fungal network. This study provides the first account of the dynamic diversity observed in the rat skin mycobiome composition, structure, and network assembly associated with postcutaneous burn injury.

中文翻译：

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与大鼠（Rattus norvegicus）深部局部皮肤烧伤相关的真菌组和网络结构的时间变化。

哺乳动物的皮肤具有多种多样的生理接口，可以抵御病原体的入侵，是抵御病原体入侵的第一道防线，并且拥有可维持上皮屏障功能和预防疾病的微生物群落。尽管广泛研究了皮肤细菌居民的动态作用，但真菌成分（菌群）的作用却被大大忽略了。结果，它们在皮肤损伤期间的影响，例如烧伤中皮肤完整性的破坏和宿主免疫防御系统的损伤，尚未明确描述。与其他住院患者相比，烧伤患者有发生难以治疗的真菌感染的高风险。为了辨别皮肤烧伤过程中真菌组和网络组件的变化，使用大鼠烫伤模型来调查健康（n = 30）（假烧伤）和皮肤烧伤（n = 24）皮肤在11天内的菌群。随着时间的推移，健康的皮肤表现出动物间异质性，而烧伤的皮肤真菌组向烧伤后第3天开始减少的动物间差异过渡到暂时稳定的群落。主要由念珠菌相对丰度的显着增加所致，尤其是在烧伤后第7天和11天，真菌种类的丰富性和烧伤皮肤的丰度降低了。与健康的大鼠皮肤真菌网络相比，大鼠皮肤真菌基因组的网络体系结构显示了网络重组，网络脆弱性增加，稳定性降低。这项研究首次说明了在大鼠皮肤中的菌群的组成，结构，