Zebrafish or Danio rerio is an established model organism for studying the genetic, neuronal and behavioral bases of diseases and for toxicology and drug screening. The embryonic and larval stages of zebrafish have been used extensively in fundamental and applied research due to advantages offered such as body transparency, small size, low cost of cultivation and high genetic homology with humans. However, the manual experimental methods used for handling and investigating this organism are limited due to their low throughput, labor intensiveness and inaccuracy in delivering external stimuli to the zebrafish while quantifying various neuronal and behavioral responses. Microfluidic and lab-on-a-chip devices have emerged as ideal technologies to overcome these challenges. In this review paper, the current microfluidic approaches for investigation of behavior and neurobiology of zebrafish at embryonic and larval stages will be reviewed. Our focus will be to provide an overview of the microfluidic methods used to manipulate (deliver and orient), immobilize and expose or inject zebrafish embryos or larvae, followed by quantification of their responses in terms of neuron activities and movement. We will also provide our opinion in terms of the direction that the field of zebrafish microfluidics is heading toward in the area of biomedical engineering.

中文翻译：

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用于胚胎和幼虫斑马鱼研究的微流控设备。

斑马鱼或达尼奥雷里奥（Danio rerio）是建立模型生物，用于研究疾病的遗传，神经元和行为基础以及毒理学和药物筛选。斑马鱼的胚胎期和幼虫期由于其具有的优势（如体透明，体积小，培育成本低以及与人类的遗传同源性高）而已在基础研究和应用研究中广泛使用。但是，用于处理和研究该生物的人工实验方法受到限制，因为它们的通量低，劳动强度大且在将外部刺激传递给斑马鱼时不准确，同时还量化了各种神经元和行为反应。微流体和芯片实验室设备已经成为克服这些挑战的理想技术。在这篇评论文章中，目前将审查斑马鱼在胚胎和幼虫阶段的行为和神经生物学研究的微流控方法。我们的重点将是概述用于操纵（传递和定向），固定和暴露或注射斑马鱼胚胎或幼虫的微流体方法，然后根据神经元的活动和运动对它们的反应进行量化。我们还将就斑马鱼微流体技术在生物医学工程领域的发展方向提供意见。然后量化他们在神经元活动和运动方面的反应。我们还将就斑马鱼微流体技术在生物医学工程领域的发展方向提供意见。然后量化他们在神经元活动和运动方面的反应。我们还将就斑马鱼微流体技术在生物医学工程领域的发展方向提供意见。