The widespread application of nanoparticles causes extensive public concern on their biological safety. However, up to date, few reports have been associated with the impact of metal nanoparticles on gut microbiota in vivo. In this study, gold nanoclusters (AuNCs) were orally administered to BALB/c mice for 7 days, and the dynamic effects of AuNCs on gut microbiota were investigated at 0 h, 4 h, 8 h, 1 d, 3 d and 7 d, respectively. Using high-throughput sequencing of V4-V5 regions of the 16S rRNA gene on the Illumina MiSeq platform, we found that gavage of AuNCs did not significantly change the gut microbiota diversity and community at the initial 3 days. Bacteroidetes, Firmicutes, Proteobacteria, Deferribacteres, Tenericutes and Actinobacteria were the six most abundant phylum in the control and AuNCs groups. However, the phylum Proteobacteria was significantly increased by AuNCs at 7 d (P < 0.05). At the genus level, Roseburia were notably decreased while Staphylococcus, Ureaplasma and Methylobacterium were dramatically increased by AuNCs at 7 d (P < 0.05). Taken together, gavage of AuNCs shifted the bacterial composition at 7 d. This is the first research to investigate the toxicity of AuNCs from the perspective of gut microbiota and opens up a new avenue for the safety or toxicity evaluation of AuNCs.

中文翻译：

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AuNCs的短期暴露对BALB / c小鼠肠道菌群的影响。

纳米粒子的广泛应用引起公众对其生物安全性的广泛关注。然而，迄今为止，鲜有报道涉及金属纳米颗粒对体内肠道菌群的影响。。在这项研究中，金纳米团（AuNCs）口服给予BALB / c小鼠7天，并在0 h，4 h，8 h，1 d，3 d和7 d研究了AuNCs对肠道菌群的动态作用。 ， 分别。使用Illumina MiSeq平台上16S rRNA基因的V4-V5区的高通量测序，我们发现管饲AuNCs在最初的3天并未显着改变肠道菌群的多样性和群落。拟杆菌，硬毛菌，变形杆菌，去铁杆菌，Tenericutes和放线菌是对照组和AuNCs组中最丰富的六个门。然而，AuNCs在7 d时门生菌群显着增加（P <0.05）。在属水平上，玫瑰葡萄球菌明显减少，而葡萄球菌，解和Methylobacterium在7 d（分别显着增加通过AuNCs P <0.05）。合计，管饲AuNCs在7 d时改变了细菌组成。这是从肠道菌群角度研究AuNCs毒性的第一项研究，为AuNCs的安全性或毒性评估开辟了一条新途径。