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Vitellogenins in the spider Parasteatoda tepidariorum - expression profile and putative hormonal regulation of vitellogenesis.
BMC Developmental Biology ( IF 1.978 ) Pub Date : 2019-03-08 , DOI: 10.1186/s12861-019-0184-x
Agata W Bednarek 1 , Marta K Sawadro 1 , Łukasz Nicewicz 1 , Agnieszka I Babczyńska 1
Affiliation  

BACKGROUND Knowledge about vitellogenesis in spiders is rudimentary. Therefore, the aim of study was to check the vitellogenin (Vg) presence in various tissues of the female spider Parasteatoda tepidariorum, determine when and where vitellogenesis starts and takes place, and the putative role of selected hormones in the vitellogenesis. RESULTS Here we show two genes encoding Vg (PtVg4 and PtVg6) in the genome of the spider P. tepidariorum. One gene PtVg4 and three subunits of Vg (250 kDa, 47 kDa and 30 kDa) are expressed in the midgut glands, ovaries and hemolymph. Heterosynthesis of the Vg in the midgut glands and autosynthesis in the ovaries were observed. Vitellogenesis begins in the last nymphal stage in the midgut glands (heterosynthesis). However, after sexual maturity is reached, Vg is also synthesized in the ovaries (autosynthesis). Changes in the PtVg4 expression level and in the Vg concentration after treatment with 20-hydroxyecdysone, a juvenile hormone analog (fenoxycarb) and an antijuvenoid compound (precocene I) were observed. Therefore, we propose a hypothetical model for the hormonal regulation of vitellogenesis in P. tepidariorum. CONCLUSIONS Our results are the first comprehensive study on spider vitellogenesis. In our opinion, this work will open discussion on the evolutionary context of possible similarities in the hormonal control of vitellogenesis between P. tepidariorum and other arthropods as well as their consequences.

中文翻译:

蜘蛛 Parasteatoda tepidariorum 中的卵黄蛋白原 - 表达谱和卵黄发生的假定激素调节。

背景技术关于蜘蛛卵黄发生的知识还很初级。因此,研究的目的是检查雌性蜘蛛 Parasteatoda tepidariorum 各个组织中卵黄蛋白原 (Vg) 的存在,确定卵黄发生开始和发生的时间和地点,以及所选激素在卵黄发生中的假定作用。结果在这里,我们展示了蜘蛛 P. tepidariorum 基因组中编码 Vg 的两个基因(PtVg4 和 PtVg6)。一种基因 PtVg4 和三个 Vg 亚基(250 kDa、47 kDa 和 30 kDa)在中肠腺、卵巢和血淋巴中表达。观察到中肠腺中 Vg 的异质合成和卵巢中的自合成。卵黄发生开始于中肠腺的最后若虫阶段(异质合成)。然而,性成熟后,Vg 也会在卵巢中合成(自合成)。观察用 20-羟基蜕皮酮、保幼激素类似物 (fenoxycarb) 和抗保幼激素化合物 (precocene I) 处理后 PtVg4 表达水平和 Vg 浓度的变化。因此,我们提出了一个关于 P. tepidariorum 卵黄发生的激素调节的假设模型。结论我们的结果是第一个关于蜘蛛卵黄发生的综合研究。我们认为,这项工作将开启关于 P. tepidariorum 和其他节肢动物之间卵黄发生的激素控制可能相似的进化背景及其后果的讨论。
更新日期:2020-04-22
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