Polyethylene glycol-poly(lactic-co-glycolic acid) (PEG-PLGA) nanoparticles coated with neutrophil membranes were fabricated to afford a bio-mimicking delivery system and achieve targeted delivery of chemotherapeutics towards malignant melanoma via systemic administration. Using celastrol as the model compound, celastrol-loaded PEG-PLGA nanoparticles coated with neutrophil membranes displayed significantly enhanced cytotoxicity and apoptosis rate in a murine melanoma cell line B16F10 compared to celastrol-loaded PEG-PLGA nanoparticles. Moreover, PEG-PLGA nanoparticles coated with neutrophil membranes exhibited significantly higher internalization efficiency in B16F10 cells than nanoparticles without membrane coating. Next, a B16F10 tumor xenograft mice model was established to explore the biodistribution profiles of PEG-PLGA nanoparticles coated with neutrophil membranes which showed remarkably prolonged blood circulation and more selective accumulation at the tumor site. Celastrol-loaded PEG-PLGA nanoparticles coated with neutrophil membranes also demonstrated greatly improved antitumor efficacy in B16F10 tumor bearing mice xenografts. Taken together, PEG-PLGA nanoparticles coated with neutrophil membranes represent a highly promising nanoscale delivery system to achieve tumor-targeted therapy following systemic administration.

中文翻译：

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生物模拟纳米颗粒用于恶性黑色素瘤的靶向治疗。

制备涂有嗜中性粒细胞膜的聚乙二醇-聚乳酸-乙醇酸共聚物（PEG-PLGA）纳米颗粒，以提供一种仿生物传递系统，并通过全身给药实现针对恶性黑色素瘤的靶向化学治疗。使用celastrol作为模型化合物，与负载celastrol的PEG-PLGA纳米颗粒相比，涂覆有嗜中性粒细胞膜的celastrol负载的PEG-PLGA纳米颗粒在鼠黑素瘤细胞系B16F10中显示出明显增强的细胞毒性和凋亡率。此外，涂有嗜中性粒细胞膜的PEG-PLGA纳米颗粒在B16F10细胞中的内在化效率要比没有膜涂层的纳米颗粒高得多。下一个，建立了B16F10肿瘤异种移植小鼠模型，以研究涂有嗜中性粒细胞膜的PEG-PLGA纳米粒子的生物分布特征，该粒子显示血液循环显着延长，并在肿瘤部位更具选择性。涂有中性粒细胞膜的负载Celastrol的PEG-PLGA纳米颗粒在携带B16F10肿瘤的小鼠异种移植物中也显示出极大的抗肿瘤功效。总而言之，涂有嗜中性粒细胞膜的PEG-PLGA纳米颗粒代表了一种高度有前途的纳米级递送系统，可在全身性给药后实现肿瘤靶向治疗。用中性粒细胞膜包被的负载Celastrol的PEG-PLGA纳米颗粒还证明在携带B16F10肿瘤的小鼠异种移植物中的抗肿瘤功效大大提高。总而言之，涂有嗜中性粒细胞膜的PEG-PLGA纳米颗粒代表了一种高度有前途的纳米级递送系统，可在全身性给药后实现肿瘤靶向治疗。用中性粒细胞膜包被的负载Celastrol的PEG-PLGA纳米颗粒还证明在携带B16F10肿瘤的小鼠异种移植物中的抗肿瘤功效大大提高。总而言之，涂有嗜中性粒细胞膜的PEG-PLGA纳米颗粒代表了一种高度有前途的纳米级递送系统，可在全身性给药后实现肿瘤靶向治疗。