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Independent, Controllable Stretch-Perfusion Bioreactor Chambers to Functionalize Cell-Seeded Decellularized Tendons.
Annals of Biomedical Engineering ( IF 3.8 ) Pub Date : 2019-04-08 , DOI: 10.1007/s10439-019-02257-6
Giuseppe Talò 1 , Daniele D'Arrigo 1 , Sergio Lorenzi 2 , Matteo Moretti 1, 3, 4 , Arianna B Lovati 1
Affiliation  

Tissue-engineered decellularized matrices can progress clinical replacement of full-thickness ruptures or tendon defects. This study develops and validates a custom-made automated bioreactor, called oscillating stretch-perfusion bioreactor (OSPB), consisting of multiple, independent culture chambers able to combine a bidirectional perfusion with a programmable, uniaxial strain to functionalize cell-seeded decellularized tendons. Decellularized tendon matrices were seeded on their surfaces and within the tendon fibers with mesenchymal stem cells. Then, they were subjected to a bidirectional perfusion and programmed stretching cycles of 15-30-60 min on-off two times per day for 7 days of culture. In vitro analyses showed viable cells, homogenously distributed on the surface of the constructs. More importantly, cell-seeded decellularized tendon grafts undergoing cyclic load in our bioreactor had a superior production and organization of newly formed collagen matrix compared to static cultured constructs. The coherency and local alignment of the new collagen deposition within the inner injected channels quantitatively supported histological findings. The designed OSPB could be considered a unique, cost-effective system able to involve multiple independently controlled chambers in terms of biological and mechanical protocols. This system allows parallel processing of several customized tendon constructs to be used as grafts to enhance the surgical repair of large tendon defects.

中文翻译:

独立的,可控制的拉伸-灌注生物反应器腔室,以功能化接种细胞的脱细胞肌腱。

组织工程化的脱细胞基质可以促进全厚度破裂或肌腱缺损的临床替代。这项研究开发并验证了一种定制的自动化生物反应器,称为振荡拉伸灌注生物反应器(OSPB),该反应器由多个独立的培养室组成,这些培养室能够将双向灌注与可编程的单轴应变相结合,以功能化接种细胞的脱细胞肌腱。将脱细胞的肌腱基质与间充质干细胞一起接种在其表面和肌腱纤维内。然后,将它们每天进行两次双向灌流和15-30-60分钟的编程拉伸循环,每天两次,共培养7天。体外分析显示,活细胞均匀地分布在构建体表面。更重要的是,与静态培养的构建体相比,在我们的生物反应器中经受循环负载的细胞播种的脱细胞腱移植物具有更高的生产和组织新形成的胶原蛋白基质的能力。内部注射通道内新胶原蛋白沉积的相干性和局部排列在数量上支持了组织学发现。设计的OSPB可以被认为是一个独特的,具有成本效益的系统,能够在生物学和机械规程方面涉及多个独立控制的腔室。该系统允许并行处理几种定制的肌腱结构以用作移植物,以增强大肌腱缺损的手术修复。内部注射通道内新胶原蛋白沉积的相干性和局部排列在数量上支持了组织学发现。设计的OSPB可以被认为是一个独特的,具有成本效益的系统,能够在生物学和机械规程方面涉及多个独立控制的腔室。该系统允许并行处理几种定制的肌腱结构以用作移植物,以增强大肌腱缺损的手术修复。内部注射通道内新胶原蛋白沉积的相干性和局部排列在数量上支持了组织学发现。设计的OSPB可以被认为是一个独特的,具有成本效益的系统,能够在生物学和机械规程方面涉及多个独立控制的腔室。该系统允许并行处理几种定制的肌腱结构以用作移植物,以增强大肌腱缺损的手术修复。
更新日期:2020-02-12
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