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A role for scavenger receptor B1 as a captor of specific fatty acids in taste buds of circumvallate papillae.
Biomedical Research ( IF 1.2 ) Pub Date : 2018-12-12 , DOI: 10.2220/biomedres.39.295
Satoshi Tsuzuki 1 , Shinhye Lee 1 , Yusaku Kimoto 2 , Tatsuya Sugawara 3 , Yuki Manabe 3 , Kazuo Inoue 1
Affiliation  

Class B scavenger receptor family members, scavenger receptor B1 (SR-B1) and cluster of differentiation 36 (CD36), are broadly expressed cell-surface proteins, both of which are believed to serve as multifaceted players in lipid and lipoprotein metabolism in mammals. Because of its presence in the apical part of taste receptor cells within circumvallate taste buds and its ability to recognise long-chain fatty acids, CD36 has been believed to participate in the sensing of the lipid species within the oral cavity. However, there have been no attempts to address whether SR-B1 has such a role to date. In this study, by reverse transcription- polymerase chain reaction analysis, we detected SR-B1 mRNA in a total RNA sample isolated from the circumvallate papillae of mouse tongue. Immunohistochemical analysis of tongue sections from the animals revealed the expression of SR-B1 protein in a population of taste bud cells of circumvallate papillae. In addition, the pattern of staining in the papillae for SR-B1 agreed closely with that for CD36 in double immunostaining analysis. We performed a cell-free in-vitro assay utilising a peptide mimic of SR-B1 and provided evidence that the receptor could recognise certain of the unsaturated long-chain fatty acids such as oleic acid. Our present findings suggest an additional role for SR-B1 as a captor of specific fatty acids in the oral cavity of mammals and contribute to expanding our knowledge of the physiological function of the receptor.

中文翻译:

清道夫受体B1在环化乳突乳头味蕾中作为特定脂肪酸的捕获剂的作用。

B类清道夫受体家族成员,清道夫受体B1(SR-B1)和分化簇36(CD36)是广泛表达的细胞表面蛋白,据信这两种蛋白均可在哺乳动物的脂质和脂蛋白代谢中发挥多方面的作用。由于其存在于周缘味蕾中味觉感受器细胞的顶端,并且具有识别长链脂肪酸的能力,因此CD36被认为参与了口腔内脂质种类的感应。但是,迄今为止,尚未尝试解决SR-B1是否具有这种作用。在这项研究中,通过逆转录-聚合酶链反应分析,我们从鼠舌的周缘乳头分离出的总RNA样品中检测到SR-B1 mRNA。从动物的舌头切片进行的免疫组织化学分析显示,SR-B1蛋白在环状乳头的味蕾细胞中表达。此外,在双重免疫染色分析中,SR-B1的乳头染色模式与CD36的模式非常吻合。我们利用SR-B1的肽模拟物进行了无细胞体外测定,并提供了证据表明该受体可以识别某些不饱和长链脂肪酸,例如油酸。我们目前的发现表明,SR-B1作为哺乳动物口腔中特定脂肪酸的捕获剂具有额外的作用,并且有助于扩大我们对受体生理功能的了解。在双重免疫染色分析中,SR-B1的乳头染色模式与CD36的模式非常吻合。我们利用SR-B1的肽模拟物进行了无细胞体外测定,并提供了证据表明该受体可以识别某些不饱和长链脂肪酸,例如油酸。我们目前的发现表明,SR-B1作为哺乳动物口腔中特定脂肪酸的捕获剂具有额外的作用,并且有助于扩大我们对受体生理功能的了解。在双重免疫染色分析中,SR-B1的乳头染色模式与CD36的模式非常吻合。我们利用SR-B1的肽模拟物进行了无细胞体外测定,并提供了证据表明该受体可以识别某些不饱和长链脂肪酸,例如油酸。我们目前的发现表明,SR-B1作为哺乳动物口腔中特定脂肪酸的捕获剂具有额外的作用,并且有助于扩大我们对受体生理功能的了解。
更新日期:2019-11-01
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