ABSTRACT Zinc deficiency and excess can result in adverse health outcomes. There is conflicting evidence regarding whether excess or deficient zinc in the diet can contribute to carcinogenicity. The objective of this study was to characterize zinc carbonate basic for use as a source of dietary zinc in a rodent toxicity and carcinogenicity study investigating the effects of zinc deficiency and excess. Because of the complex chemistries of zinc carbonate basic compounds, inconsistent nomenclature, and literature and reference spectra gaps, it was necessary to use multiple analytical techniques, including Karl Fischer titration, combustion analysis, inductively coupled plasma–optical emission spectrometry, X-ray diffraction, infrared spectroscopy, X-ray fluorescence spectrometry, and thermogravimetric analysis to characterize the test article. Based on the collective evidence and through the process of elimination, the test article was found to be composed mainly of zinc carbonate basic with zinc oxide as a minor component. The zinc content was determined to be 56.6% (w/w) with heavy metals such as arsenic, cadmium, mercury, and lead below the limit of quantitation of less than or equal to 0.01%. The test material was stable at ambient temperature. Based on the work described in this manuscript, the test article was suitable for use as a source of zinc in studies of deficiency and excess in the diet.

中文翻译：

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在研究膳食缺乏或过量影响的啮齿动物研究中碱式碳酸锌作为锌源的表征

摘要锌缺乏和过量会导致不良的健康结果。关于饮食中过量或缺乏锌是否会导致致癌性，存在相互矛盾的证据。本研究的目的是在啮齿动物毒性和致癌性研究中表征用作膳食锌来源的碱性碳酸锌，研究锌缺乏和过量的影响。由于碳酸锌碱性化合物的化学性质复杂、命名不一致、文献和参考光谱有差距，因此有必要使用多种分析技术，包括卡尔费休滴定、燃烧分析、电感耦合等离子体发射光谱、X 射线衍射、红外光谱、X 射线荧光光谱和热重分析来表征测试物品。根据集体证据并通过排除过程，发现测试物品主要由碱式碳酸锌和少量氧化锌组成。锌含量测定为 56.6% (w/w)，砷、镉、汞和铅等重金属低于定量限小于或等于 0.01%。测试材料在环境温度下是稳定的。根据本手稿中描述的工作，该测试物品适合用作研究饮食中缺乏和过量的锌来源。并且铅低于小于或等于 0.01% 的定量限。测试材料在环境温度下是稳定的。根据本手稿中描述的工作，测试物品适用于研究饮食中的缺乏和过量的锌源。并且铅低于小于或等于 0.01% 的定量限。测试材料在环境温度下是稳定的。根据本手稿中描述的工作，该测试物品适合用作研究饮食中缺乏和过量的锌来源。