Anthropogenic environmental change has led to unprecedented rates of species extinction, presenting a major threat to global biodiversity. Among vertebrates, amphibians have been most severely impacted, with an estimated 41% of species now threatened with extinction. In response to this biodiversity crisis, a moral and ethical obligation exists to implement proactive interventionist conservation actions to assist species recovery and decelerate declines. Conservation breeding programs have been successfully established for several threatened amphibian species globally, aiming to prevent species' extinction by maintaining genetically representative assurance colonies ex situ while providing individuals for population augmentation, translocation, and reestablishment in situ. Reproductive technologies have enormous potential to enhance the propagation and genetic management of threatened species. In this review, we discuss the role of reproductive technologies in amphibian conservation breeding programs and summarize technological advancements in amphibian hormone therapies, gamete storage, and artificial fertilization.

中文翻译：

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生殖技术在两栖动物保护育种计划中的作用。

人为的环境变化导致物种灭绝的速度空前，对全球生物多样性构成了重大威胁。在脊椎动物中，两栖动物受到的影响最为严重，目前估计有41％的物种濒临灭绝。为了应对这种生物多样性危机，存在着道德和伦理义务，采取积极的干预主义保护行动，以帮助物种恢复和减缓衰退。全球已经成功地为几种濒临灭绝的两栖动物建立了保护育种计划，其目的是通过在原地维持具有遗传代表性的保证性殖民地，同时为个体提供种群增加，易位和原地重建，来防止物种灭绝。生殖技术在增强受威胁物种的繁殖和遗传管理方面具有巨大潜力。在这篇综述中，我们讨论了生殖技术在两栖动物保护育种计划中的作用，并总结了两栖动物激素治疗，配子储藏和人工受精方面的技术进步。