In this paper, a miRNA-based quartz crystal microbalance (QCM) biosensor was fabricated and used to the rapid and effective sensing of miRNA. The specific hybridization between probe miRNA and different selected miRNAs (miR-27a, miR-27b, and Let-7a) cause a different interaction mode, thus display different frequency change and response patterns in the QCM sensor, which were used to detect miR-27a and miR-27b. The selective sensing of miR-27a in mixed miRNA solution was also achieved. This miRNA-based QCM biosensor has the advantages of real-time, label-free, and short cycle detection.

中文翻译：

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用于通过响应模式识别miRNA的miRNA修饰的QCM传感器的组装。

本文制备了基于miRNA的石英晶体微天平（QCM）生物传感器，并将其用于快速有效的miRNA传感。探针miRNA与不同的选定miRNA（miR-27a，miR-27b和Let-7a）之间的特异性杂交会导致不同的相互作用模式，从而在QCM传感器中显示出不同的频率变化和响应模式，用于检测miR- 27a和miR-27b。还实现了混合miRNA溶液中miR-27a的选择性检测。这种基于miRNA的QCM生物传感器具有实时，无标记和短周期检测的优势。