BACKGROUND With increased social pressure, individuals face a high risk of depression. Subsequently, depression affects cognitive behaviour and negatively impacts daily life. Fortunately, the Traditional Chinese Medicine Jia Wei Xiao Yao (JWXY) capsule is effective in reducing depression and improving cognitive behaviour. METHODS The constituents of JWXY capsule were identified by ultra-performance liquid chromatography and quadrupole time-of-flight mass spectrometry analyses. We analysed behaviours of depression-like zebrafish in the novel tank with an automatic 3D video-tracking system and conducted the colour preference test, as well detected physiological changes after sertraline and JWXY capsule treatments. RESULTS Both sertraline and JWXY capsule rescued the decreased locomotive behaviour and depression phenotype of zebrafish caused by reserpine. JWXY capsule especially improved the inhibited exploratory behaviour caused by reserpine. In addition, with the onset of depressive behaviour, zebrafish exhibited alterations in cognitive behaviour as indicated by colour preference changes. However, compared with sertraline, JWXY capsule was more efficaciously in rescuing this change in the colour preference pattern. Moreover, an increased level of cortisol, increased expression of tyrosine hydroxylase (TH) and decreased monoamine neurotransmitters, including serotonin (5-HT) and noradrenaline, were involved in the depressive behaviours. In addition, sertraline and JWXY capsule rescued the depressive phenotype and cognitive behaviour of zebrafish by altering the levels of endogenous cortisol and monoamine neurotransmitters. CONCLUSIONS JWXY capsule was more effectively than sertraline in rescuing reserpine-induced depression and cognitive disorder in zebrafish. Potentially, our study can provide new insights into the clinical treatment of depression and the mechanism of action of JWXY capsule.

中文翻译：

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舍曲林和中药（TCM）逆转利血平引起的斑马鱼抑郁症和认知障碍。

背景技术随着社会压力的增加，个人面临抑郁的高风险。随后，抑郁会影响认知行为，并对日常生活产生负面影响。幸运的是，中药佳味小药（JWXY）胶囊可有效减轻抑郁症并改善认知行为。方法通过超高效液相色谱和四极杆飞行时间质谱分析法鉴定JWXY胶囊的成分。我们使用自动3D视频跟踪系统分析了新型水箱中凹陷状斑马鱼的行为，并进行了颜色偏好测试，以及检测了舍曲林和JWXY胶囊处理后的生理变化。结果舍曲林和JWXY胶囊均能挽救利血平引起的斑马鱼机车行为减少和抑郁表型。JWXY胶囊特别改善了利血平引起的探索性行为抑制。此外，随着抑郁行为的发作，斑马鱼表现出认知行为的改变，如颜色偏好的变化所表明的。但是，与舍曲林相比，JWXY胶囊在挽救颜色偏爱模式中的这种变化方面更有效。此外，抑郁行为与皮质醇水平升高，酪氨​​酸羟化酶（TH）表达增加和单胺神经递质（包括5-羟色胺（5-HT）和去甲肾上腺素）降低有关。此外，舍曲林和JWXY胶囊通过改变内源性皮质醇和单胺类神经递质的水平，拯救了斑马鱼的抑郁表型和认知行为。结论JWXY胶囊在挽救利血平引起的抑郁症和斑马鱼认知障碍方面比舍曲林更有效。潜在地，我们的研究可以为抑郁症的临床治疗和JWXY胶囊的作用机理提供新的见解。