A dual modality microscopy with the highest imaging resolution reported so far based on reflection-mode photoacoustic and confocal fluorescence is presented in this study. The unique design of the imaging head of the microscope makes it highly convenient for scalable high-resolution imaging by simply switching the optical objectives. The submicron resolution performance of the system is demonstrated via in vivo imaging of zebrafish, normal mouse ear, and a xenograft tumor model inoculated in the mouse ear. The imaging results confirm that the presented dual-modality microscopy imaging system could play a vital role in observing model organism, studying tumor angiogenesis and assessment of antineoplastic drugs.

在这项研究中提出了迄今为止报道的基于反射模式光声和共聚焦荧光的具有最高成像分辨率的双模态显微镜。显微镜成像头的独特设计使其通过简单地切换光学物镜就非常适合可扩展的高分辨率成像。该系统的亚微米分辨率性能是通过斑马鱼，正常小鼠耳朵和在小鼠耳朵中接种的异种移植肿瘤模型的体内成像证明的。成像结果证实，所提出的双模式显微镜成像系统在观察模型生物，研究肿瘤血管生成和评估抗肿瘤药物方面可以发挥至关重要的作用。