A combination of liquid chromatography, ion mobility spectrometry, mass spectrometry, and database searching techniques were used to characterize the proteomes of four biological replicates of adult Drosophila melanogaster heads at seven time points across their lifespans. Based on the detection of tryptic peptides, the identities of 1281 proteins were determined. An estimate of the abundance of each protein, based on the three most intense peptide ions, shows that the quantified species vary in concentration over a factor of ~103. Compared to initial studies in the field of Drosophila proteomics, our current results show an eight-fold higher temporal protein coverage with increased quantitative accuracy. Across the lifespan, we observe a range of trends in the abundance of different proteins, including: an increase in abundance of proteins involved in oxidative phosphorylation, and the tricarboxylic acid cycle; a decrease in proteasomal proteins, as well as ribosomal proteins; and, many types of proteins, which remain relatively unchanged. For younger flies, proteomes are relatively similar within their age group. For older flies, proteome similarity decreases within their age group. These combined results illustrate a correlation between increasing age and decreasing proteostasis.

中文翻译：

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衰老果蝇头部的蛋白质组变化

液相色谱、离子迁移谱、质谱和数据库搜索技术的组合被用来表征成年黑腹果蝇头部在其整个生命周期中的七个时间点的四个生物重复的蛋白质组。基于胰蛋白酶肽的检测，确定了1281种蛋白质的身份。根据三种最强的肽离子对每种蛋白质的丰度进行估计，表明量化物质的浓度变化超过约 103 倍。与果蝇蛋白质组学领域的初步研究相比，我们目前的结果显示，时间蛋白质覆盖率提高了八倍，并且定量准确性更高。在整个生命周期中，我们观察到不同蛋白质丰度的一系列趋势，包括：参与氧化磷酸化和三羧酸循环的蛋白质丰度增加；蛋白酶体蛋白和核糖体蛋白减少；以及许多类型的蛋白质，它们保持相对不变。对于年轻的果蝇，蛋白质组在其年龄组中相对相似。对于年龄较大的果蝇，其年龄组内的蛋白质组相似性降低。这些综合结果说明了年龄增加和蛋白质稳态减少之间的相关性。