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Effect of single-dose ibuprofen on follicular fluid levels of interleukins in poor responders undergoing in vitro fertilization.
Systems Biology in Reproductive Medicine ( IF 2.4 ) Pub Date : 2018-12-20 , DOI: 10.1080/19396368.2018.1557761
Laurice Bou Nemer 1 , Haolin Shi 1 , Bruce Richard Carr 1 , Ruth Ann Word 1 , Orhan Bukulmez 1
Affiliation  

Premature follicular rupture during in vitro fertilization (IVF) is a well-known culprit for cycle cancellation. We sought to evaluate whether a single oral dose of ibuprofen will have an effect on the follicular fluid (FF) levels of inflammatory markers involved in ovulation. This is a prospective within-subjects study following nine patients undergoing IVF. Every patient underwent a first cycle of minimal stimulation IVF followed by a second cycle using the same stimulation protocol, except one oral dose of ibuprofen 800 mg was administered 15–18 h post-trigger injection. FF was obtained during oocyte retrievals of both cycles and analyzed for levels of selected inflammatory markers. A total of 27 cytokines and 9 matrix metalloproteinases (MMPs) were tested. Results demonstrate significantly decreased levels of interleukin (IL)-6, IL-8, granulocyte-colony stimulating factor (G-CSF), eotaxin, MMP3, MMP7, MMP12, and MMP13 in FF of cycles where ibuprofen was administered. However, other cytokines levels, such IL-1 and vascular endothelial growth factor (VEGF), were similar with or without ibuprofen. Levels of MMPs described to be involved in ovulation, namely MMP-2 and MMP-9, were either undetectable or unchanged by ibuprofen, respectively. In conclusion, our data show that one dose of ibuprofen administered orally the day after trigger injection revealed a significant impact on the FF inflammatory milieu.

Abbreviations: IVF: in vitro fertilization; MMP: matrix metalloproteinase; IL: interleukins; FF: follicular fluid; VEGF: vascular endothelial growth factor; NSAIDS: non-steroidal anti-inflammatories; POR: poor ovarian response; AMH: anti-Mullerian hormone; TAFC: total antral follicle count; HMG: human menopausal gonadotropin; hCG: human chorionic gonadotropin; COX: cyclooxygenase enzymes; PGH2: prostaglandin H2; RANTES: regulated on activation, normal T expressed and secreted; NF-κb: nuclear factor kappa-light-chain-enhancer of activated B cells



中文翻译:

单剂量布洛芬对接受体外受精的不良反应者卵泡液中白介素水平的影响。

体外滤泡过早破裂受精(IVF)是导致循环取消的罪魁祸首。我们试图评估单次口服布洛芬是否会对卵泡液(FF)参与排卵的炎症标志物水平产生影响。这是对9名接受试管婴儿的患者进行的前瞻性受试者内部研究。除了触发后注射15–18 h给予800 mg布洛芬一次口服剂量外,每个患者均经历了第一轮IVF最小刺激,然后进行了第二轮相同的刺激方案。在两个周期的卵母细胞取回过程中均获得FF,并分析所选炎症标志物的水平。总共测试了27种细胞因子和9种基质金属蛋白酶(MMP)。结果显示白介素(IL)-6,IL-8,粒细胞集落刺激因子(G-CSF),给予布洛芬的FF周期中的嗜酸性粒细胞趋化因子,MMP3,MMP7,MMP12和MMP13。但是,在有或没有布洛芬的情况下,其他细胞因子水平(例如IL-1和血管内皮生长因子(VEGF))相似。被描述为与排卵有关的MMPs的水平,即MMP-2和MMP-9,分别被布洛芬检测不到或保持不变。总之,我们的数据显示,触发注射后第二天口服一剂布洛芬对FF炎症环境有显着影响。被布洛芬检测不到或保持不变。总之,我们的数据显示,触发注射后第二天口服一剂布洛芬对FF炎症环境有显着影响。被布洛芬检测不到或保持不变。总之,我们的数据显示,触发注射后第二天口服一剂布洛芬对FF炎症环境有显着影响。

缩写: IVF:体外受精;MMP:基质金属蛋白酶;IL:白介素;FF:卵泡液;VEGF:血管内皮生长因子;NSAIDS:非甾体类抗炎药;POR:卵巢反应不良;AMH:抗穆勒激素;TAFC:总的肛门卵泡计数;HMG:人类更年期促性腺激素;hCG:人绒毛膜促性腺激素;COX:环氧合酶;PGH2:前列腺素H2;RANTES:受激活,正常T表达和分泌的调节;NF-κb:活化的B细胞的核因子κ轻链增强剂

更新日期:2018-12-20
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