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Cloning, localization and bioinformatics analysis of a gene encoding an odorant-binding protein (OBP) in Anoplophora glabripennis (Motschulsky).
Invertebrate Neuroscience Pub Date : 2018-08-31 , DOI: 10.1007/s10158-018-0215-5
Yu Li 1 , Huien Li 2 , Zhigang Wang 1, 2 , Danyang Gao 1 , Kun Xiao 1 , Aihua Yan 1, 2
Affiliation  

Anoplophora glabripennis (Motschulsky) has an advanced and complicated olfactory system to identify hosts, mates and spawning locations, and odorant-binding proteins (OBPs) play a key role by binding to volatile materials from different hosts. The full-length cDNA sequence of an OBP, AglaOBP, was cloned by RACE from an antenna cDNA library, and the protein structure and function were predicted by bioinformatics analysis. Gene temporal and spatial expression was detected by real-time qPCR. AglaOBP had distinctive sequence, location and expression profiles compared with other OBPs of A. glabripennis, as it was found in different tissues, and the highest expression was in the elytrums. The possible physiological functions of this OBP were discussed. These findings help elucidate the physiology of this pest and provide a new potential target for pest control.

中文翻译:

Alaboren glabripennis(Motschulsky)中编码气味结合蛋白(OBP)的基因的克隆,定位和生物信息学分析。

Anoplophora glabripennis(Motschulsky)具有先进而复杂的嗅觉系统,可识别宿主,伴侣和产卵位置,而气味结合蛋白(OBP)通过与来自不同宿主的挥发性物质结合而发挥关键作用。通过RACE从触角cDNA文库中克隆了OBP的全长cDNA序列AglaOBP,并通过生物信息学分析预测了蛋白质的结构和功能。通过实时qPCR检测基因的时空表达。与A的其他OBP相比,AglaOBP具有独特的序列,位置和表达谱。牛油果,因为它在不同的组织中被发现,并且最高的表达是在鞘翅中。讨论了该OBP的可能的生理功能。这些发现有助于阐明该害虫的生理学,并为控制害虫提供了新的潜在目标。
更新日期:2018-08-31
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