Neurite outgrowth is a morphological marker of neuronal differentiation and neuroregeneration, and the process includes four essential phases, namely initiation, elongation, guidance and cessation. Intrinsic and extrinsic signaling molecules seem to involve morphological changes of neurite outgrowth via various cellular signaling cascades phase transition. Although mechanisms associated with neurite outgrowth have been studied extensively, little is known about how phase transition is regulated during neurite outgrowth. 5-HT has long been studied with regard to its relationship to neurite outgrowth in invertebrate and vertebrate culture systems, and many studies have suggested 5-HT inhibits neurite elongation and growth cone motility, in particular, at the growing parts of neurite such as growth cones and filopodia. However, the underlying mechanisms need to be investigated. In this study, we investigated roles of 5-HT on neurite outgrowth using single serotonergic neurons C1 isolated from Helisoma trivolvis. We observed that 5-HT delayed phase transitions from initiation to elongation of neurite outgrowth. This study for the first time demonstrated that 5-HT has a critical role in phase-controlling mechanisms of neurite outgrowth in neuronal cell cultures.

中文翻译：

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5-HT在已确定的5-羟色胺能神经元C1旋毛虫Helisoma trivolvis神经突增生的相变中的作用。

神经突生长是神经元分化和神经再生的形态学标志，该过程包括四个基本阶段，即起始，延伸，引导和停止。内部和外部信号分子似乎通过各种细胞信号级联相变而涉及神经突生长的形态变化。尽管已广泛研究了与神经突生长相关的机制，但对神经突生长期间如何调控相变的了解甚少。长期以来，人们一直在研究5-HT与无脊椎动物和脊椎动物培养系统中神经突生长的关系，许多研究表明5-HT尤其是在神经突的生长部位（例如生长）抑制了神经突的伸长和锥体生长。视锥细胞和丝状伪足。然而，潜在的机制需要调查。在这项研究中，我们使用分离自大鼠的单个血清素能神经元C1，研究了5-HT对神经突生长的作用。旋毛虫。我们观察到5-HT延迟了从神经突向外生长的开始到伸长的相变。这项研究首次证明，5-HT在神经元细胞培养物中神经突生长的相位控制机制中起着关键作用。