Protein kinase A (PKA), also known as cAMP dependent protein kinase, is an essential component of many signaling pathways, many of which regulate key developmental processes. Inactive PKA is a tetrameric holoenzyme, comprised of two catalytic (PRKAC), and two regulatory subunits. Upon cAMP binding, the catalytic subunits are released and thereby activated. There are multiple isoforms of PKA catalytic subunits, but their individual roles are not well understood. In order to begin studying their roles in zebrafish development, it is first necessary to identify the spatial and temporal expression profiles for each prkac subunit. Here we evaluate the expression profiles for the four zebrafish prkacs: prkacαa, αb, βa, and βb, at key developmental time points: 24, 48 and 72 h post fertilization. We show that zebrafish prkacs are expressed throughout the developing nervous system, each showing unique expression patterns. This body of work will inform future functional studies into the roles of PKA during development.

蛋白激酶A（PKA），也称为cAMP依赖性蛋白激酶，是许多信号通路的重要组成部分，其中许多调节关键的发育过程。非活性PKA是四聚体全酶，由两个催化（PRKAC）和两个调节亚基组成。在cAMP结合后，催化亚基被释放并因此被活化。PKA催化亚基有多种同工型，但对其各自的作用尚不甚了解。为了开始研究它们在斑马鱼发育中的作用，首先必须确定每个prkac亚基的时空表达谱。在这里，我们评估了四种斑马鱼prkacs的表达谱：prkacαa，αb，βa和βb，在关键的发育时间点：受精后24、48和72小时。我们显示，斑马鱼prkacs在整个发育中的神经系统中表达，每个显示出独特的表达模式。该工作将为将来的功能研究提供有关PKA在开发过程中的作用的信息。