Abstract The aim of this study was to evaluate whether lycopene-loaded liposomes (L-LYC) could interfere with the antitumor efficacy and cardiotoxicity of doxorubicin (DOX). L-LYC were prepared by a thin-film hydration method to overcome the instability, insolubility, and low bioavailability of lycopene. The mean diameter and morphology of the liposomes were determined by dynamic light scattering and transmission electron microscopy, respectively, and then, in vitro cytotoxicity and in vivo antitumor activity were determined to evaluate the effects of L-LYC and their combination with DOX. Finally, we evaluated whether L-LYC could decrease the DOX-induced cardiotoxicity in vivo. The results showed that the particle size of L-LYC appeared uniform, and the average diameter was approximately 160.4 nm. Compared with DOX treatment alone, the combination of L-LYC and DOX showed significantly increased cytotoxicity in vitro and decreased the tumor size in B16 melanoma-bearing mice in vivo. Furthermore, the DOX-induced cardiotoxicity was clearly relieved in combination with L-LYC. The overall findings indicated that L-LYC have a great potential for improving the therapeutic efficacy and attenuating the cardiotoxicity of the chemotherapy drug DOX.

中文翻译：

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多柔比星与番茄红素脂质体联合使用可增强抗肿瘤功效并减弱心脏毒性

摘要 本研究的目的是评估装载番茄红素的脂质体 (L-LYC) 是否会干扰阿霉素 (DOX) 的抗肿瘤功效和心脏毒性。L-LYC是通过薄膜水合法制备的，以克服番茄红素的不稳定性、不溶性和生物利用度低的问题。脂质体的平均直径和形态分别通过动态光散射和透射电子显微镜测定，然后测定体外细胞毒性和体内抗肿瘤活性，以评估 L-LYC 及其与 DOX 组合的效果。最后，我们评估了 L-LYC 是否可以降低 DOX 诱导的体内心脏毒性。结果表明，L-LYC的粒径均匀，平均粒径约为160.4 nm。与单独使用 DOX 治疗相比，L-LYC 和 DOX 的组合在体外显示出显着增加的细胞毒性，并在体内减小了携带 B16 黑色素瘤的小鼠的肿瘤大小。此外，DOX 诱导的心脏毒性与 L-LYC 组合明显减轻。总体研究结果表明，L-LYC 在提高化疗药物 DOX 的治疗效果和减轻心脏毒性方面具有巨大潜力。