ABSTRACT Long noncoding RNAs (lncRNAs) regulate the expression of mRNA and can affect various biological processes and phenotypes. Currently, studies of lncRNAs in cattle are under way, but their exact function for several tissues has not yet been established. Hanwoo cattle (Bos taurus coreanae) have inhabited the Korean peninsula for about 6000 years and are one of the representative domesticated animals in Korea. As a result of intensive breeding, the meat of Hanwoo cattle is high in marbling content and is preferred by Koreans and other East Asian people. In this study, the expression of lncRNAs was identified in 36 samples from skeletal muscle and three adipose tissues (intramuscular, subcutaneous, and omental) of nine Hanwoo individuals. We identified 76 tissue-specific lncRNAs for each of the four tissues using the differences in expression levels. Through QTL information, we could identify 12 lncRNAs associated with shear force and six lncRNAs associated with body weight, which are two important traits in the Hanwoo population breeding strategy. By the physical position comparison of lncRNA and Bovine transcripts information, we could identify 11 lncRNAs that were in bovine transcripts, and four of the 11 genes related to transcripts of lncRNAs were biologically associated with muscle function. We believe this Hanwoo lncRNAs study will help reveal the lncRNA role in the physiological mechanisms of these four tissues.

中文翻译：

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韩宇肉牛肌肉和脂肪组织中长链非编码RNA的比较

摘要 长非编码 RNA (lncRNA) 调节 mRNA 的表达，并能影响各种生物过程和表型。目前，对牛 lncRNA 的研究正在进行中，但其对多种组织的确切功能尚未确定。韩牛牛（Bos taurus coreanae）在朝鲜半岛栖息了约6000年，是韩国代表性的家养动物之一。由于集约化饲养，韩牛牛的肉质富含大理石花纹，深受韩国人和其他东亚人的喜爱。在这项研究中，在来自 9 名 Hanwoo 个体的骨骼肌和三种脂肪组织（肌内、皮下和网膜）的 36 个样本中鉴定了 lncRNA 的表达。我们利用表达水平的差异，为四种组织中的每一种组织鉴定了 76 个组织特异性 lncRNA。通过QTL信息，我们可以识别出12个与剪切力相关的lncRNA和6个与体重相关的lncRNA，这是Hanwoo群体育种策略中的两个重要性状。通过lncRNA和牛转录本信息的物理位置比较，我们可以识别出牛转录本中的11个lncRNA，并且与lncRNA转录本相关的11个基因中有4个与肌肉功能有生物学相关。我们相信这项 Hanwoo lncRNA 研究将有助于揭示 lncRNA 在这四种组织的生理机制中的作用。