In this study, we sought to evaluate the effects of dietary Bacillus amyloliquefaciens (B. amyloliquefaciens) BLCC1-0238 supplementation on laying performance, egg quality, antioxidant enzyme activities, reproductive hormone, and immunity of laying hens. A total of 240 Hy-Line Brown laying hens (28 weeks old) were randomly divided into four groups, and three replicates per group (n = 20 per replicate). The control group was fed a standard basal diet, and the three treatment groups were provided the basal diet supplemented with either 0.01%, 0.03%, or 0.06% B. amyloliquefaciens BLCC1-0238 (2 × 10¹⁰ CFU/g), respectively. Hens were allowed 2 weeks to acclimate prior to initiation of the 8-week experiment. It was observed that dietary supplementation with 0.01% or 0.03% B. amyloliquefaciens BLCC1-0238 significantly increased egg production and egg mass. However, no significant differences in feed intake, egg weight, and feed conversion ratio among the four groups were observed. Different levels of B. amyloliquefaciens BLCC1-0238 supplementation also significantly increased egg shell strength and thickness. With respect to the levels of reproductive hormones in the hens, B. amyloliquefaciens BLCC1-0238 supplementation significantly reduced serum adrenal cortical hormone (ACTH) levels, while increasing estradiol (E2) and follicle-stimulating hormone (FSH) secretion in the treatment groups compared to the control group. Relative to the control group, supplementation with 0.03% and 0.06% B. amyloliquefaciens BLCC1-0238 was observed to significantly increase serum glutathione S-transferase (GST) concentration, and supplementation significantly reduced serum IL-1 and IL-6 levels, whereas IL-4 levels increased for all concentrations tested. In conclusion, supplementation of a basal chicken diet with B. amyloliquefaciens BLCC1-0238 can improve laying performance and egg quality through the reduction of stress responses, up-regulation of growth hormones, and supporting immunity in laying hens.

中文翻译：

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淀粉芽孢杆菌BLCC1-0238可以通过增强产蛋鸡的免疫力和调节其生殖激素来有效地提高产蛋性能和蛋品质。

在这项研究中，我们试图评估饮食的影响，解淀粉芽孢杆菌（B.杆菌）BLCC1-0238补充对产蛋性能，蛋品质，抗氧化酶活性，生殖激素，和蛋鸡的免疫力。蛋鸡（28周龄）总共240海兰褐的随机分为四组，并且每组三个重复（Ñ 每个重复= 20）。对照组饲以标准基础饲料，以及三个治疗组提供补充有0.01％，0.03％，或0.06％的基础饮食解淀粉芽孢杆菌BLCC1-0238（2×10 ¹⁰ CFU / g）。在开始8周的实验之前，让母鸡适应2周。观察到，膳食补充0.01％或0.03％的解淀粉芽孢杆菌BLCC1-0238显着提高了产蛋量和蛋重。然而，在四组中，在采食量，蛋重和饲料转化率上没有观察到显着差异。不同水平的解淀粉芽孢杆菌BLCC1-0238补充剂也显着增加了蛋壳强度和厚度。关于母鸡中的生殖激素水平，解淀粉芽孢杆菌与对照组相比，BLCC1-0238补充剂在治疗组中显着降低了血清肾上腺皮质激素（ACTH）水平，同时增加了雌二醇（E2）和促卵泡激素（FSH）分泌。相对于对照组，补充0.03％和0.06％的解淀粉芽孢杆菌BLCC1-0238可显着增加血清谷胱甘肽S-转移酶（GST）的浓度，并且补充显着降低血清IL-1和IL-6的水平，而IL所有测试浓度的-4水平增加。总之，在淀粉基础芽孢杆菌中补充基础鸡饮食 BLCC1-0238可以通过减少应激反应，上调生长激素并支持蛋鸡免疫来提高蛋鸡生产性能和蛋品质。